Cloned (Comment) | Organism |
---|---|
expression in COS-7 and in CHO cells, secretion from recombinant COS-7 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amastatin | - |
Homo sapiens | |
Zn2+ | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.28 | - |
Leu-4-nitroanilide | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | zinc-metallopeptidase, contains the HEXXH motif | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
1 * 106000, recombinant enzyme, SDS-PAGE | Homo sapiens |
120000 | - |
recombinant enzyme from CHO cells, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin II + H2O | Homo sapiens | - |
angiotensin IV + ? | through angiotensin III | ? | |
kallidin + H2O | Homo sapiens | i.e. LRPPGFSPFR | Leu + bradykinin | i.e. RPPGFSPFR | ? | |
additional information | Homo sapiens | broad substrate specificity towards naturally occurring peptide hormones, while the enzyme hydrolyzes synthetic substrate in vitro with specificity for leucine, enzyme plays a role in regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from CHO cells, 5fold to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipocyte | - |
Homo sapiens | - |
kidney | cortex | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.192 | - |
purified recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin II + H2O | - |
Homo sapiens | angiotensin IV + ? | through angiotensin III | ? | |
dynorphin A + H2O | - |
Homo sapiens | ? | - |
? | |
kallidin + H2O | i.e. LRPPGFSPFR | Homo sapiens | Leu + bradykinin | i.e. RPPGFSPFR | ? | |
Leu-4-nitroanilide + H2O | - |
Homo sapiens | Leu + 4-nitroaniline | - |
? | |
additional information | broad substrate specificity towards naturally occurring peptide hormones, while the enzyme hydrolyzes synthetic substrate in vitro with specificity for leucine, enzyme plays a role in regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney | Homo sapiens | ? | - |
? | |
Neurokinin A + H2O | - |
Homo sapiens | ? | - |
? | |
Neuromedin B + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 106000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
A-LAP | - |
Homo sapiens |
adipocyte-derived leucine aminopeptidase | - |
Homo sapiens |
aminopeptidase I | - |
Homo sapiens |
aminopeptidase II | - |
Homo sapiens |
Aminopeptidase III | - |
Homo sapiens |
cathepsin III | - |
Homo sapiens |
cytosol aminopeptidase | - |
Homo sapiens |
FTBL protein | - |
Homo sapiens |
L-leucine aminopeptidase | - |
Homo sapiens |
leucinamide aminopeptidase | - |
Homo sapiens |
leucinaminopeptidase | - |
Homo sapiens |
leucine aminopeptidase | - |
Homo sapiens |
leucyl peptidase | - |
Homo sapiens |
More | enzyme belongs to the peptidase family M17, enzyme belongs to the metallopeptidase family M1 | Homo sapiens |
peptidase S | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Homo sapiens |