Cloned (Comment) | Organism |
---|---|
expression of a DNA library in Salmonella typhimurium, DNA and sequence determination and analysis, expression of the full length enzyme in Salmonella typhimurium strain TN5179 | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
K310V | increased specificty for Leu as Xaa, no activity with Asp-Leu in opposite to the wild-type enzyme | Salmonella enterica |
R402W | reduced activity with Asp-Leu compared to the wild-type enzyme | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | complete inhibition at 37° and 1 mM within 20 min | Salmonella enterica | |
EDTA | inactivation can be restored by divalent cations | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Asp-Phe | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
0.3 | - |
Asp-Tyr | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
0.4 | - |
Asp-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
0.5 | - |
Asp-Leu-Gly | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
0.6 | - |
Asp-His | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
0.9 | - |
Glu-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
1 | - |
Leu-Tyr | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
1.8 | - |
Asp-Gln | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
3 | - |
Asp-Ile-Gly-Gly | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
4 | - |
Leu-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
6 | - |
Asp-Leu-Lys | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | stimulates, optimal at 50 mM | Salmonella enterica | |
Mg2+ | - |
Salmonella enterica | |
additional information | enzyme is a metallopeptidase | Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
gel filtration | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Leu + H2O | Salmonella enterica | - |
Ala + Leu | - |
? | |
Asn-Leu + H2O | Salmonella enterica | - |
Asn + Leu | - |
? | |
Asp-Leu + H2O | Salmonella enterica | - |
Asp + Leu | - |
? | |
Cys-Leu + H2O | Salmonella enterica | - |
Cys + Leu | - |
? | |
Glu-Leu + H2O | Salmonella enterica | - |
Glu + Leu | - |
? | |
His-Leu + H2O | Salmonella enterica | - |
His + Leu | - |
? | |
Leu-Leu + H2O | Salmonella enterica | - |
Leu + Leu | - |
? | |
Met-Leu + H2O | Salmonella enterica | - |
Met + Leu | - |
? | |
additional information | Salmonella enterica | no growth on QL, FL, YL, WL, IL, VL, KL, GL, RL, and PL | ? | - |
? | |
Ser-Leu + H2O | Salmonella enterica | - |
Ser + Leu | - |
? | |
Thr-Leu + H2O | Salmonella enterica | - |
Thr + Leu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | Q9RF52 | serovar typhimurium | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from strain TN5179, 2.8fold | Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of the wild-type enzyme with Leu-Xaa, Asp-Xaa, Glu-Xaa and tripeptide substrates, overview | Salmonella enterica |
1 | - |
mutant K310V, substrate Asp-Leu | Salmonella enterica |
11 | - |
wild-type enzyme, substrate Ile-Leu and Val-Leu | Salmonella enterica |
13 | - |
wild-type enzyme, substrate Ser-Leu | Salmonella enterica |
18 | - |
wild-type enzyme, substrate Thr-Leu and Ala-Leu | Salmonella enterica |
25 | - |
wild-type enzyme, substrate Trp-Leu | Salmonella enterica |
32 | - |
wild-type enzyme, substrate Tyr-Leu | Salmonella enterica |
39 | - |
wild-type enzyme, substrate Phe-Leu | Salmonella enterica |
49 | - |
wild-type enzyme, substrate Gln-Leu | Salmonella enterica |
63 | - |
mutant R402W, substrate Asp-Leu | Salmonella enterica |
126 | - |
wild-type enzyme, substrate Asn-Leu | Salmonella enterica |
128 | - |
wild-type enzyme, substrate Cys-Leu | Salmonella enterica |
129 | 223 | purified recombinant wild-type enzyme, substrate Leu-Leu | Salmonella enterica |
137 | - |
mutant R402W, substrate Leu-Leu | Salmonella enterica |
156 | - |
wild-type enzyme, substrate His-Leu | Salmonella enterica |
164 | - |
wild-type enzyme, substrate Met-Leu | Salmonella enterica |
239 | - |
purified recombinant wild-type enzyme, substrate Asp-Leu | Salmonella enterica |
310 | - |
mutant K310V, substrate Leu-Leu | Salmonella enterica |
408 | - |
wild-type enzyme, substrate Glu-Leu | Salmonella enterica |
550 | - |
wild-type enzyme, substrate Asp-Leu | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Leu + H2O | - |
Salmonella enterica | Ala + Leu | - |
? | |
Asn-Leu + H2O | - |
Salmonella enterica | Asn + Leu | - |
? | |
Asp-Gln + H2O | - |
Salmonella enterica | Asp + Gln | - |
? | |
Asp-His + H2O | - |
Salmonella enterica | Asp + His | - |
? | |
Asp-Ile-Gly-Gly + H2O | - |
Salmonella enterica | Asp + Ile-Gly-Gly | - |
? | |
Asp-Leu + H2O | - |
Salmonella enterica | Asp + Leu | - |
? | |
Asp-Leu-Gly + H2O | - |
Salmonella enterica | Asp + Leu-Gly | - |
? | |
Asp-Leu-Lys + H2O | - |
Salmonella enterica | Asp + Leu-Lys | - |
? | |
Asp-Phe + H2O | - |
Salmonella enterica | Asp + Phe | - |
? | |
Asp-Tyr + H2O | - |
Salmonella enterica | Asp + Tyr | - |
? | |
Cys-Leu + H2O | - |
Salmonella enterica | Cys + Leu | - |
? | |
Gln-Leu + H2O | - |
Salmonella enterica | Gln + Leu | - |
? | |
Glu-Leu + H2O | - |
Salmonella enterica | Glu + Leu | - |
? | |
His-Leu + H2O | - |
Salmonella enterica | His + Leu | - |
? | |
Ile-Leu + H2O | - |
Salmonella enterica | Ile + Leu | - |
? | |
Leu-Leu + H2O | - |
Salmonella enterica | Leu + Leu | - |
? | |
Leu-Tyr + H2O | - |
Salmonella enterica | Leu + Tyr | - |
? | |
Met-Leu + H2O | - |
Salmonella enterica | Met + Leu | - |
? | |
additional information | substrate specificity study, overview, Lys-Leu, Gly-Leu, Arg-Leu, and Pro-Leu are poor or no substrates | Salmonella enterica | ? | - |
? | |
additional information | no growth on QL, FL, YL, WL, IL, VL, KL, GL, RL, and PL | Salmonella enterica | ? | - |
? | |
Phe-Leu + H2O | - |
Salmonella enterica | Phe + Leu | - |
? | |
Ser-Leu + H2O | - |
Salmonella enterica | Ser + Leu | - |
? | |
Thr-Leu + H2O | - |
Salmonella enterica | Thr + Leu | - |
? | |
Trp-Leu + H2O | - |
Salmonella enterica | Trp + Leu | - |
? | |
Tyr-Leu + H2O | - |
Salmonella enterica | Tyr + Leu | - |
? | |
Val-Leu + H2O | - |
Salmonella enterica | Val + Leu | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 46360-46362, mass spectrometry and DNA sequence calculation | Salmonella enterica |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Salmonella enterica |
PepB | - |
Salmonella enterica |
peptidase B | - |
Salmonella enterica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
rapid loss of activity of crude cell extract, but the purified enzyme is stable | Salmonella enterica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
Asp-Leu-Lys | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
26 | - |
Asp-Ile-Gly-Gly | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
351 | - |
Leu-Tyr | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
418 | - |
Asp-His | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
500 | - |
Asp-Phe | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
500 | - |
Asp-Tyr | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
666 | - |
Asp-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
761 | - |
Asp-Gln | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
866 | - |
Glu-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
918 | - |
Leu-Leu | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica | |
950 | - |
Asp-Leu-Gly | pH 8.5, 37°C, wild-type enzyme | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | - |
Salmonella enterica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Salmonella enterica |