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Literature summary for 3.4.11.1 extracted from

  • Mathew, Z.; Knox, T.M.; Miller, C.G.
    Salmonella enterica serovar Typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids (2000), J. Bacteriol., 182, 3383-3393.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of a DNA library in Salmonella typhimurium, DNA and sequence determination and analysis, expression of the full length enzyme in Salmonella typhimurium strain TN5179 Salmonella enterica

Protein Variants

Protein Variants Comment Organism
K310V increased specificty for Leu as Xaa, no activity with Asp-Leu in opposite to the wild-type enzyme Salmonella enterica
R402W reduced activity with Asp-Leu compared to the wild-type enzyme Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
bestatin complete inhibition at 37° and 1 mM within 20 min Salmonella enterica
EDTA inactivation can be restored by divalent cations Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
Asp-Phe pH 8.5, 37°C, wild-type enzyme Salmonella enterica
0.3
-
Asp-Tyr pH 8.5, 37°C, wild-type enzyme Salmonella enterica
0.4
-
Asp-Leu pH 8.5, 37°C, wild-type enzyme Salmonella enterica
0.5
-
Asp-Leu-Gly pH 8.5, 37°C, wild-type enzyme Salmonella enterica
0.6
-
Asp-His pH 8.5, 37°C, wild-type enzyme Salmonella enterica
0.9
-
Glu-Leu pH 8.5, 37°C, wild-type enzyme Salmonella enterica
1
-
Leu-Tyr pH 8.5, 37°C, wild-type enzyme Salmonella enterica
1.8
-
Asp-Gln pH 8.5, 37°C, wild-type enzyme Salmonella enterica
3
-
Asp-Ile-Gly-Gly pH 8.5, 37°C, wild-type enzyme Salmonella enterica
4
-
Leu-Leu pH 8.5, 37°C, wild-type enzyme Salmonella enterica
6
-
Asp-Leu-Lys pH 8.5, 37°C, wild-type enzyme Salmonella enterica

Metals/Ions

Metals/Ions Comment Organism Structure
KCl stimulates, optimal at 50 mM Salmonella enterica
Mg2+
-
Salmonella enterica
additional information enzyme is a metallopeptidase Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
gel filtration Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Ala-Leu + H2O Salmonella enterica
-
Ala + Leu
-
?
Asn-Leu + H2O Salmonella enterica
-
Asn + Leu
-
?
Asp-Leu + H2O Salmonella enterica
-
Asp + Leu
-
?
Cys-Leu + H2O Salmonella enterica
-
Cys + Leu
-
?
Glu-Leu + H2O Salmonella enterica
-
Glu + Leu
-
?
His-Leu + H2O Salmonella enterica
-
His + Leu
-
?
Leu-Leu + H2O Salmonella enterica
-
Leu + Leu
-
?
Met-Leu + H2O Salmonella enterica
-
Met + Leu
-
?
additional information Salmonella enterica no growth on QL, FL, YL, WL, IL, VL, KL, GL, RL, and PL ?
-
?
Ser-Leu + H2O Salmonella enterica
-
Ser + Leu
-
?
Thr-Leu + H2O Salmonella enterica
-
Thr + Leu
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica Q9RF52 serovar typhimurium
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from strain TN5179, 2.8fold Salmonella enterica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity of the wild-type enzyme with Leu-Xaa, Asp-Xaa, Glu-Xaa and tripeptide substrates, overview Salmonella enterica
1
-
mutant K310V, substrate Asp-Leu Salmonella enterica
11
-
wild-type enzyme, substrate Ile-Leu and Val-Leu Salmonella enterica
13
-
wild-type enzyme, substrate Ser-Leu Salmonella enterica
18
-
wild-type enzyme, substrate Thr-Leu and Ala-Leu Salmonella enterica
25
-
wild-type enzyme, substrate Trp-Leu Salmonella enterica
32
-
wild-type enzyme, substrate Tyr-Leu Salmonella enterica
39
-
wild-type enzyme, substrate Phe-Leu Salmonella enterica
49
-
wild-type enzyme, substrate Gln-Leu Salmonella enterica
63
-
mutant R402W, substrate Asp-Leu Salmonella enterica
126
-
wild-type enzyme, substrate Asn-Leu Salmonella enterica
128
-
wild-type enzyme, substrate Cys-Leu Salmonella enterica
129 223 purified recombinant wild-type enzyme, substrate Leu-Leu Salmonella enterica
137
-
mutant R402W, substrate Leu-Leu Salmonella enterica
156
-
wild-type enzyme, substrate His-Leu Salmonella enterica
164
-
wild-type enzyme, substrate Met-Leu Salmonella enterica
239
-
purified recombinant wild-type enzyme, substrate Asp-Leu Salmonella enterica
310
-
mutant K310V, substrate Leu-Leu Salmonella enterica
408
-
wild-type enzyme, substrate Glu-Leu Salmonella enterica
550
-
wild-type enzyme, substrate Asp-Leu Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Leu + H2O
-
Salmonella enterica Ala + Leu
-
?
Asn-Leu + H2O
-
Salmonella enterica Asn + Leu
-
?
Asp-Gln + H2O
-
Salmonella enterica Asp + Gln
-
?
Asp-His + H2O
-
Salmonella enterica Asp + His
-
?
Asp-Ile-Gly-Gly + H2O
-
Salmonella enterica Asp + Ile-Gly-Gly
-
?
Asp-Leu + H2O
-
Salmonella enterica Asp + Leu
-
?
Asp-Leu-Gly + H2O
-
Salmonella enterica Asp + Leu-Gly
-
?
Asp-Leu-Lys + H2O
-
Salmonella enterica Asp + Leu-Lys
-
?
Asp-Phe + H2O
-
Salmonella enterica Asp + Phe
-
?
Asp-Tyr + H2O
-
Salmonella enterica Asp + Tyr
-
?
Cys-Leu + H2O
-
Salmonella enterica Cys + Leu
-
?
Gln-Leu + H2O
-
Salmonella enterica Gln + Leu
-
?
Glu-Leu + H2O
-
Salmonella enterica Glu + Leu
-
?
His-Leu + H2O
-
Salmonella enterica His + Leu
-
?
Ile-Leu + H2O
-
Salmonella enterica Ile + Leu
-
?
Leu-Leu + H2O
-
Salmonella enterica Leu + Leu
-
?
Leu-Tyr + H2O
-
Salmonella enterica Leu + Tyr
-
?
Met-Leu + H2O
-
Salmonella enterica Met + Leu
-
?
additional information substrate specificity study, overview, Lys-Leu, Gly-Leu, Arg-Leu, and Pro-Leu are poor or no substrates Salmonella enterica ?
-
?
additional information no growth on QL, FL, YL, WL, IL, VL, KL, GL, RL, and PL Salmonella enterica ?
-
?
Phe-Leu + H2O
-
Salmonella enterica Phe + Leu
-
?
Ser-Leu + H2O
-
Salmonella enterica Ser + Leu
-
?
Thr-Leu + H2O
-
Salmonella enterica Thr + Leu
-
?
Trp-Leu + H2O
-
Salmonella enterica Trp + Leu
-
?
Tyr-Leu + H2O
-
Salmonella enterica Tyr + Leu
-
?
Val-Leu + H2O
-
Salmonella enterica Val + Leu
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 46360-46362, mass spectrometry and DNA sequence calculation Salmonella enterica

Synonyms

Synonyms Comment Organism
LAP
-
Salmonella enterica
PepB
-
Salmonella enterica
peptidase B
-
Salmonella enterica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Salmonella enterica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
rapid loss of activity of crude cell extract, but the purified enzyme is stable Salmonella enterica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13
-
Asp-Leu-Lys pH 8.5, 37°C, wild-type enzyme Salmonella enterica
26
-
Asp-Ile-Gly-Gly pH 8.5, 37°C, wild-type enzyme Salmonella enterica
351
-
Leu-Tyr pH 8.5, 37°C, wild-type enzyme Salmonella enterica
418
-
Asp-His pH 8.5, 37°C, wild-type enzyme Salmonella enterica
500
-
Asp-Phe pH 8.5, 37°C, wild-type enzyme Salmonella enterica
500
-
Asp-Tyr pH 8.5, 37°C, wild-type enzyme Salmonella enterica
666
-
Asp-Leu pH 8.5, 37°C, wild-type enzyme Salmonella enterica
761
-
Asp-Gln pH 8.5, 37°C, wild-type enzyme Salmonella enterica
866
-
Glu-Leu pH 8.5, 37°C, wild-type enzyme Salmonella enterica
918
-
Leu-Leu pH 8.5, 37°C, wild-type enzyme Salmonella enterica
950
-
Asp-Leu-Gly pH 8.5, 37°C, wild-type enzyme Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5
-
Salmonella enterica

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
Salmonella enterica