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Literature summary for 3.3.2.9 extracted from

  • Li, N.; Zhang, Y.; Feng, H.
    Biochemical characterization and transcriptional analysis of the epoxide hydrolase from white-rot fungus Phanerochaete chrysosporium (2009), Acta Biochim. Biophys. Sin. (Shanghai), 41, 638-647 .
    View publication on PubMed
Search for more literature for 3.3.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Phanerodontia chrysosporium

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 5 mM, 14% residual activity Phanerodontia chrysosporium
H2O2 5 mM, 79% residual activity Phanerodontia chrysosporium
Hg2+ 5 mM, 36% residual activity Phanerodontia chrysosporium
additional information not inhibitory: EDTA Phanerodontia chrysosporium
Zn2+ 5 mM, 41% residual activity Phanerodontia chrysosporium

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 5 mM, 108% of initial activity Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 PAGE Phanerodontia chrysosporium

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 10% (v/v), 100% of initial activity Phanerodontia chrysosporium
dimethyl sulfoxide 10% (v/v), 88% of initial activity Phanerodontia chrysosporium
Ethanol 10% (v/v), 92% of initial activity Phanerodontia chrysosporium
formamide 10% (v/v), 82% of initial activity Phanerodontia chrysosporium
Glycerol 10% (v/v), 115% of initial activity Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium B0FSQ0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1,2-epoxybutane + H2O very limited preference for hydrolysis of the (R)-enantiomer Phanerodontia chrysosporium (2R)-butane-1,2-diol
-
 ?
(R)-epichlorhydrin + H2O very limited preference for hydrolysis of the (S)-enantiomer Phanerodontia chrysosporium (R)-3-chloro-1,2-propanediol
-
 ?
(R)-glycidyl tosylate + H2O very limited preference for hydrolysis of the (R)-enantiomer Phanerodontia chrysosporium (2R)-4-(4-methylbenzene-1-sulfonyl)butane-1,2,4-triol
-
 ?
(R)-styrene oxide + H2O preferential hydrolysis of the (R)-enantiomer Phanerodontia chrysosporium (R)-styrene glycol
-
 ?
(S)-1,2-epoxybutane + H2O very limited preference for hydrolysis of the (R)-enantiomer Phanerodontia chrysosporium (2S)-butane-1,2-diol
-
 ?
(S)-epichlorhydrin + H2O very limited preference for hydrolysis of the (S)-enantiomer Phanerodontia chrysosporium (S)-3-chloro-1,2-propanediol
-
 ?
(S)-glycidyl tosylate + H2O very limited preference for hydrolysis of the (R)-enantiomer Phanerodontia chrysosporium (2S)-4-(4-methylbenzene-1-sulfonyl)butane-1,2,4-triol
-
 ?
additional information the enzyme exhibits broad hydrolytic activity toward several racemic epoxides including styrene oxide, epichlorohydrin, and 1,2-epoxybutane Phanerodontia chrysosporium ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37700, calculated, 1 * 42000, SDS-PAGE of recombinant His-tagged protein Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
Eha
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 substrate racemic styrene oxide Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 substrate racemic styrene oxide Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
8 10
-
 Phanerodontia chrysosporium

Expression

Organism Comment Expression
Phanerodontia chrysosporium transcripts of Eha can be detected under the ligninolytic and nonligninolytic conditions as well as induced by anthracene additional information