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Literature summary for 3.2.2.28 extracted from

  • Sung, J.S.
    Mosbaugh, D.W.: Escherichia coli double-strand uracil-DNA glycosylase: involvement in uracil-mediated DNA base excision repair and stimulation of activity by endonuclease IV (2000), Biochemistry, 39, 10224-10235.
    View publication on PubMed
Search for more literature for 3.2.2.28

Activating Compound

Activating Compound Comment Organism Structure
endonuclease IV endonuclease IV stimulates Dug activity by enhancing the rate and extent of uracil excision by promoting dissociation of Dug from the apyrimidinic-site/G-containing 34-mer. Catalytically active endonuclease IV is required to mediate Dug turnover Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information double-strand uracil-DNA glycosylase is insensitive to uracil-DNA glycosylase inhibitor protein, i.e. Ugi Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18670
-
 matrix-assisted laser desorption-ionization mass spectrometry Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,N4-ethenocytosine-mismatched double-stranded DNA + H2O Dug is active on duplex oligonucleotides (34-mers) that contain site-specific 3,N4-ethenocytosine/G, and 3,N4-ethenocytosine/A mismatches Escherichia coli 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
 ?
additional information activity is not detected on DNA containing a T/G mispair or single-stranded DNA containing either a site-specific uracil or 3,N4-ethenocytosine residue. Endonuclease IV stimulates Dug activity by enhancing the rate and extent of uracil excision by promoting dissociation of Dug from the apyrimidinic-site/G-containing 34-mer. Catalytically active endonuclease IV is required to mediate Dug turnover Escherichia coli ?
-
 ?
uracil-mismatched double-stranded DNA + H2O Dug is active on duplex oligonucleotides (34-mers) that contain site-specific U/G or U/A mismatches. Dug excises a near stoichiometric amount of uracil from U/G-containing oligonucleotide substrate. The lack of turnover is the result of strong binding by Dug to the reaction product apyrimidinic-site Escherichia coli uracil + double-stranded DNA with abasic site
-
 ?

Synonyms

Synonyms Comment Organism
double-strand uracil-DNA glycosylase
-
 Escherichia coli
Dug
-
 Escherichia coli