Cloned (Comment) | Organism |
---|---|
gene Rv1259, DNA and amino aid sequence determination and analysis, cloning and overexpression of His6-tagged wild-type and mutant enzymes in Escherichia coli strains | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutants by insertion of kanamycin resistance marker in the UdgB active site motif GQDPY, phenotypes, overview | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | UdgB inhibition by dsDNA containing AP-site in the uracil-containing single-nucleotide bulge, UgdB is insensitive against UDG inhibition protein from Bacillus subtilis and shows no inhibition by uracil | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | A5U6Y7 | gene Rv1259 encoding UdgB | - |
Mycobacterium tuberculosis H37Ra / ATCC 25177 | A5U6Y7 | gene Rv1259 encoding UdgB | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strains by nickel affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | UdgB substrate specificity with diverse DNA oligomers, overview, the enzyme excises ethenocytosine and hypoxanthine from dsDNA, in addition to uracil present as a single-nucleotide bulge in dsDNA, but excision of 5-OH-C, dihydroxyuracil, and epsilonA is undetectable, MtuUdgB does not excise uracil from SSU9 ssDNA, mechanism of action, overview | Mycobacterium tuberculosis | ? | - |
? | |
additional information | UdgB substrate specificity with diverse DNA oligomers, overview, the enzyme excises ethenocytosine and hypoxanthine from dsDNA, in addition to uracil present as a single-nucleotide bulge in dsDNA, but excision of 5-OH-C, dihydroxyuracil, and epsilonA is undetectable, MtuUdgB does not excise uracil from SSU9 ssDNA, mechanism of action, overview | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
double stranded DNA specific UDG | - |
Mycobacterium tuberculosis |
dsDNA specific UDG | - |
Mycobacterium tuberculosis |
More | UdgB is a family 5 UDG | Mycobacterium tuberculosis |
UdgB | - |
Mycobacterium tuberculosis |
uracil DNA glycosylase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified UdgB, stable up to, MtuUdgB is thermo-tolerant | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |