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Literature summary for 3.2.2.28 extracted from

  • Srinath, T.; Bharti, S.K.; Varshney, U.
    Substrate specificities and functional characterization of a thermo-tolerant uracil DNA glycosylase (UdgB) from Mycobacterium tuberculosis (2007), DNA Repair, 6, 1517-1528.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Rv1259, DNA and amino aid sequence determination and analysis, cloning and overexpression of His6-tagged wild-type and mutant enzymes in Escherichia coli strains Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information construction of mutants by insertion of kanamycin resistance marker in the UdgB active site motif GQDPY, phenotypes, overview Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
additional information UdgB inhibition by dsDNA containing AP-site in the uracil-containing single-nucleotide bulge, UgdB is insensitive against UDG inhibition protein from Bacillus subtilis and shows no inhibition by uracil Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis A5U6Y7 gene Rv1259 encoding UdgB
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Mycobacterium tuberculosis H37Ra / ATCC 25177 A5U6Y7 gene Rv1259 encoding UdgB
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Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strains by nickel affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UdgB substrate specificity with diverse DNA oligomers, overview, the enzyme excises ethenocytosine and hypoxanthine from dsDNA, in addition to uracil present as a single-nucleotide bulge in dsDNA, but excision of 5-OH-C, dihydroxyuracil, and epsilonA is undetectable, MtuUdgB does not excise uracil from SSU9 ssDNA, mechanism of action, overview Mycobacterium tuberculosis ?
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?
additional information UdgB substrate specificity with diverse DNA oligomers, overview, the enzyme excises ethenocytosine and hypoxanthine from dsDNA, in addition to uracil present as a single-nucleotide bulge in dsDNA, but excision of 5-OH-C, dihydroxyuracil, and epsilonA is undetectable, MtuUdgB does not excise uracil from SSU9 ssDNA, mechanism of action, overview Mycobacterium tuberculosis H37Ra / ATCC 25177 ?
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?

Synonyms

Synonyms Comment Organism
double stranded DNA specific UDG
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Mycobacterium tuberculosis
dsDNA specific UDG
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Mycobacterium tuberculosis
More UdgB is a family 5 UDG Mycobacterium tuberculosis
UdgB
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Mycobacterium tuberculosis
uracil DNA glycosylase
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Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified UdgB, stable up to, MtuUdgB is thermo-tolerant Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mycobacterium tuberculosis