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Literature summary for 3.2.2.27 extracted from

  • Assefa, N.; Niiranen, L.; Willassen, N.; Smalås, A.; Moe, E.
    Thermal unfolding studies of cold adapted uracil-DNA N-glycosylase (UNG) from Atlantic cod (Gadus morhua). A comparative study with human UNG (2012), Comp. Biochem. Physiol. B, 161, 60-68.
    View publication on PubMed
Search for more literature for 3.2.2.27

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli NR8052 cells Gadus morhua
expressed in Escherichia coli NR8052 cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant D183G/K302R, hanging drop vapor diffusion method, using 0.1 M imidazole/maleate pH 7.8, 50 mM NaCl, 41 mM ammonium sulfate and 18% (w/v) PEG4000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D183G the mutant shows a slight increase in stability with concomitant reduction in the enzyme activity Homo sapiens
D183G/K302R the mutant shows a slight increase in stability with concomitant reduction in the enzyme activity Homo sapiens
G183D the mutation causes a reduction in the enzyme activity but no increase in stability Gadus morhua
G183D/R302K the mutation causes a reduction in the enzyme activity but no increase in stability Gadus morhua

General Stability

General Stability Organism
HEPES pH 7.5, Tris pH 7.5, and imidazole pH 8.0 are the most stabilizing buffers Homo sapiens
Tris pH 7.5 and 8.0, and HEPES pH 7.5 are the most stabilizing buffers, Gadus morhua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.5 and 15°C Gadus morhua
0.0008
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.4 and 37°C Gadus morhua
0.00157
-
 uracil-mismatched single-stranded DNA mutant enzyme G183G, at pH 7.4 and 37°C Homo sapiens
0.0021
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.0022
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.5 and 15°C Homo sapiens
0.00265
-
 uracil-mismatched single-stranded DNA mutant enzyme G183G/K302R, at pH 7.4 and 37°C Homo sapiens
0.004
-
 uracil-mismatched single-stranded DNA mutant enzyme G183D/R302K, at pH 7.4 and 37°C Gadus morhua
0.0083
-
 uracil-mismatched single-stranded DNA mutant enzyme G183D, at pH 7.4 and 37°C Gadus morhua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-mismatched single-stranded DNA + H2O Gadus morhua
-
 uracil + single-stranded DNA with abasic site
-
 ?
uracil-mismatched single-stranded DNA + H2O Homo sapiens
-
 uracil + single-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Gadus morhua
-
-
-
Homo sapiens P13051
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, SP Sepharose column chromatography and gel filtration Gadus morhua
Q-Sepharose column chromatography, SP Sepharose column chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil-mismatched single-stranded DNA + H2O
-
 Gadus morhua uracil + single-stranded DNA with abasic site
-
 ?
uracil-mismatched single-stranded DNA + H2O
-
 Homo sapiens uracil + single-stranded DNA with abasic site
-
 ?

Subunits

Subunits Comment Organism
? x * about 28000, SDS-PAGE Gadus morhua
? x * about 28000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
UNG
-
 Gadus morhua
UNG
-
 Homo sapiens
uracil-DNA N-glycosylase
-
 Gadus morhua
uracil-DNA N-glycosylase
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45.1
-
 melting temperature Gadus morhua
53.9
-
 melting temperature Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.5 and 15°C Homo sapiens
4.7
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.5 and 15°C Gadus morhua
7.7
-
 uracil-mismatched single-stranded DNA mutant enzyme G183G, at pH 7.5 and 37°C Homo sapiens
9.8
-
 uracil-mismatched single-stranded DNA mutant enzyme G183D, at pH 7.5 and 37°C Gadus morhua
10.8
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.5 and 37°C Homo sapiens
13.6
-
 uracil-mismatched single-stranded DNA mutant enzyme G183D/R302K, at pH 7.5 and 37°C Gadus morhua
16.9
-
 uracil-mismatched single-stranded DNA mutant enzyme G183G/K302R, at pH 7.5 and 37°C Homo sapiens
20.7
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.5 and 37°C Gadus morhua

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1100
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.5 and 15°C Homo sapiens
4917
-
 uracil-mismatched single-stranded DNA mutant enzyme G183G, at pH 7.4 and 37°C Homo sapiens
5150
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.4 and 37°C Homo sapiens
6367
-
 uracil-mismatched single-stranded DNA mutant enzyme G183G/K302R, at pH 7.4 and 37°C Homo sapiens
10100
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.5 and 15°C Gadus morhua
11880
-
 uracil-mismatched single-stranded DNA mutant enzyme G183D, at pH 7.4 and 37°C Gadus morhua
24980
-
 uracil-mismatched single-stranded DNA wild type enzyme, at pH 7.4 and 37°C Gadus morhua
36000
-
 uracil-mismatched single-stranded DNA mutant enzyme G183D/R302K, at pH 7.4 and 37°C Gadus morhua