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Literature summary for 3.2.2.27 extracted from

  • Kawai, A.; Higuchi, S.; Tsunoda, M.; Nakamura, K.T.; Miyamoto, S.
    Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7 (2012), Acta Crystallogr. Sect. F, 68, 1102-1105.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour diffusion method, deletion mutant enzyme and deletion mutant enzyme complexed with uracil are crystallized and analyzed by X-ray crystallography. The crystals are found to belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 52.2, b = 52.3, c = 74.7 A and a = 52.1, b = 52.2, c = 74.1 A for the apoenzyme and the enzyme complexed with uracil, respectively Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q96YD0
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Sulfurisphaera tokodaii 7 Q96YD0
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Purification (Commentary)

Purification (Comment) Organism
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Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
STK_22380 locus name Sulfurisphaera tokodaii