Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron-sulfur cluster | the [4Fe-4S]-binding cluster is important for the DNA binding and catalytic activity | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, SDS-PAGE | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | Methanocaldococcus jannaschii | cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase repairs this kind of DNA damage | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | Methanocaldococcus jannaschii DSM 2661 | cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase repairs this kind of DNA damage | uracil + double-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58829 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58829 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-oxoguanine-mismatched double-stranded DNA + H2O | the enzyme effciently removes uracil from both single- and double-stranded DNA. Activity with oligonucleotide duplexes containing 8-oxoguanine is 5fold lower than that of oligonucleotide duplex containing U:T | Methanocaldococcus jannaschii | 8-oxoguanine + double-stranded DNA with abasic site | - |
? | |
8-oxoguanine-mismatched double-stranded DNA + H2O | the enzyme effciently removes uracil from both single- and double-stranded DNA. Activity with oligonucleotide duplexes containing 8-oxoguanine is 5fold lower than that of oligonucleotide duplex containing U:T | Methanocaldococcus jannaschii DSM 2661 | 8-oxoguanine + double-stranded DNA with abasic site | - |
? | |
8-oxoguanine-mismatched single-stranded DNA + H2O | the enzyme effciently removes uracil from both single- and double-stranded DNA | Methanocaldococcus jannaschii | 8-oxoguanine + single-stranded DNA with abasic site | - |
? | |
8-oxoguanine-mismatched single-stranded DNA + H2O | the enzyme effciently removes uracil from both single- and double-stranded DNA | Methanocaldococcus jannaschii DSM 2661 | 8-oxoguanine + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase repairs this kind of DNA damage | Methanocaldococcus jannaschii | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | the enzyme effciently removes uracil from both single- and double-stranded DNA. Insignificant differences in activity level on the mismatched duplex substrates U:T, U:C, U:G and U:A. oligonucleotide substrates containing 3-mA, 7-mG and an apurinic/apyrimidinic site are not hydrolyzed by the enzyme | Methanocaldococcus jannaschii | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase repairs this kind of DNA damage | Methanocaldococcus jannaschii DSM 2661 | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | the enzyme effciently removes uracil from both single- and double-stranded DNA. Insignificant differences in activity level on the mismatched duplex substrates U:T, U:C, U:G and U:A. oligonucleotide substrates containing 3-mA, 7-mG and an apurinic/apyrimidinic site are not hydrolyzed by the enzyme | Methanocaldococcus jannaschii DSM 2661 | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | the enzyme effciently removes uracil from both single- and double-stranded DNA | Methanocaldococcus jannaschii | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | the enzyme effciently removes uracil from both single- and double-stranded DNA | Methanocaldococcus jannaschii DSM 2661 | uracil + single-stranded DNA with abasic site | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
MjUDG | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Methanocaldococcus jannaschii |
55 | 65 | - |
Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 65 | the enzyme is more active at 65°C than at 37°C | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
30 min, stable | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Methanocaldococcus jannaschii |