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Literature summary for 3.2.2.27 extracted from

  • Knavelsrud, I.; Moen, M.; Grosvik, K.; Haugland, G.; Birkeland, N.; Klungland, A.; Leiros, I.; Bjelland, S.
    The hyperthermophilic euryarchaeon Archaeoglobus fulgidus repairs uracil by single-nucleotide replacement (2010), J. Bacteriol., 192, 5755-5766.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.27

Cloned(Commentary)

Cloned (Comment) Organism
-
 Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 uracil-mismatched double-stranded DNA pH 7.5, 80°C, cell extract (wild-type enzyme) Archaeoglobus fulgidus
0.0087
-
 uracil-mismatched double-stranded DNA pH 7.5, 80°C, recombinant enzyme Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24600
-
 x * 24600, SDS-PAGE Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28007
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O the enzyme is the principal and probably only glycosylase in Aeroglobus fulgidus that removes uracil incorporated opposite adenine in DNA during replication. The enzyme excises uracil more efficiently when it is opposite guanine than when it is opposite adenine Archaeoglobus fulgidus uracil + double-stranded DNA with abasic site
-
 ?

Subunits

Subunits Comment Organism
? x * 24600, SDS-PAGE Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
Afung protein
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.048
-
 uracil-mismatched double-stranded DNA pH 7.5, 80°C, recombinant enzyme Archaeoglobus fulgidus
1.8
-
 uracil-mismatched double-stranded DNA pH 7.5, 80°C, cell extract (wild-type enzyme) Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Archaeoglobus fulgidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.5
-
 uracil-mismatched double-stranded DNA pH 7.5, 80°C, recombinant enzyme Archaeoglobus fulgidus
600
-
 uracil-mismatched double-stranded DNA pH 7.5, 80°C, cell extract (wild-type enzyme) Archaeoglobus fulgidus