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Literature summary for 3.2.2.27 extracted from

  • Bharti, S.K.; Varshney, U.
    Analysis of the impact of a uracil DNA glycosylase attenuated in AP-DNA binding in maintenance of the genomic integrity in Escherichia coli (2010), Nucleic Acids Res., 38, 2291-2301.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information allelic exchange of ung with ung::kan, ungY66H:amp or ungY66W:amp alleles shows 5fold, 3.0fold, and 2.0fold, respectively, increase in mutation frequencies, widening of the substrate binding pocket can lead to aquirement of thymine DNA glycosylase activity, overview Escherichia coli
Y66H the mutant shows 170fold reduced uracil excision activity compared to the wild-type enzyme, but like the wild-type protein, it is susceptible to inhibition by uracil and AP-DNA Escherichia coli
Y66W the mutant shows 7fold reduced uracil excision activity compared to the wild-type enzyme, and lacks TDG activity. The Y66W protein is moderately compromised and attenuated in binding to AP-DNA. The Y66W mutant maintains strict specificity for uracil excision from DNA, but it is recalcitrant to inhibition by uracil and AP-DNA Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ugi competitive binding to wild-type and mutant enzymes, overview Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P12295
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Ung shows strict specificity for uracil excision activity Escherichia coli ?
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?

Subunits

Subunits Comment Organism
More modeling of thymine in the active site pocket of Ung, overview Escherichia coli

Synonyms

Synonyms Comment Organism
UNG
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Escherichia coli
uracil DNA glycosylase
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Escherichia coli

General Information

General Information Comment Organism
metabolism uracil DNA glycosylase initiates the uracil excision repair pathway Escherichia coli