Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | allelic exchange of ung with ung::kan, ungY66H:amp or ungY66W:amp alleles shows 5fold, 3.0fold, and 2.0fold, respectively, increase in mutation frequencies, widening of the substrate binding pocket can lead to aquirement of thymine DNA glycosylase activity, overview | Escherichia coli |
Y66H | the mutant shows 170fold reduced uracil excision activity compared to the wild-type enzyme, but like the wild-type protein, it is susceptible to inhibition by uracil and AP-DNA | Escherichia coli |
Y66W | the mutant shows 7fold reduced uracil excision activity compared to the wild-type enzyme, and lacks TDG activity. The Y66W protein is moderately compromised and attenuated in binding to AP-DNA. The Y66W mutant maintains strict specificity for uracil excision from DNA, but it is recalcitrant to inhibition by uracil and AP-DNA | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ugi | competitive binding to wild-type and mutant enzymes, overview | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P12295 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ung shows strict specificity for uracil excision activity | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | modeling of thymine in the active site pocket of Ung, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
UNG | - |
Escherichia coli |
uracil DNA glycosylase | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | uracil DNA glycosylase initiates the uracil excision repair pathway | Escherichia coli |