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Literature summary for 3.2.2.27 extracted from
- Association between the herpes simplex virus-1 DNA polymerase and uracil DNA glycosylase (2010), J. Biol. Chem., 285, 27664-27672.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| UL2 is cloned downstream of the CMV immediate-early promoter as a V5-tagged protein by inserting the UL2 open reading frame, amplified by PCR from HSV-1 strain KOS genomic DNA, into pcDNA3.1D/V5-His-TOPO, expression in Spodoptera frugiperda cells | Human alphaherpesvirus 1 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | UL2 associates with the viral replisome. UL2 and the HSV-1 DNA polymerase catalytic subunit UL30 co-localize to viral prereplicative sites | Human alphaherpesvirus 1 | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Human alphaherpesvirus 1 | viral uracil DNA glycosylase, UL2, in conjunction with the HSV-1 DNA polymerase catalytic subunit, UL30, cellular AP endonuclease and DNA ligase IIIalpha/XRCC1, perform uracil-initiated base excision repair. UL30 exhibits apurinic/apyrimidinic and 5'-deoxyribose phosphate lyase activities. UL2 and UL30 co-localize to viral prereplicative sites. The interaction between HSV-1 proteins UL2 and Pol occurs in HSV-1 infected cells | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Human alphaherpesvirus 1 | - |
HSV-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant UL2 from Spodoptera frugiperda cells by gel filtration and two different steps of anion exchange chromatography | Human alphaherpesvirus 1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | viral uracil DNA glycosylase, UL2, in conjunction with the HSV-1 DNA polymerase catalytic subunit, UL30, cellular AP endonuclease and DNA ligase IIIalpha/XRCC1, perform uracil-initiated base excision repair. UL30 exhibits apurinic/apyrimidinic and 5'-deoxyribose phosphate lyase activities. UL2 and UL30 co-localize to viral prereplicative sites. The interaction between HSV-1 proteins UL2 and Pol occurs in HSV-1 infected cells | Human alphaherpesvirus 1 | ? | - |
? | |
| additional information | cleavage of oligonucleotide PBAZ7 which contains a U at position 17 | Human alphaherpesvirus 1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UL2 | - |
Human alphaherpesvirus 1 |
| uracil DNA glycosylase | - |
Human alphaherpesvirus 1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Human alphaherpesvirus 1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Human alphaherpesvirus 1 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | base excision repair is required to maintain genome stability as a means to counter the accumulation of unusual bases and to protect from the loss of DNA bases | Human alphaherpesvirus 1 |
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