Cloned (Comment) | Organism |
---|---|
gene ung-1, expression of the N-terminally GST-tagged enzyme in Escherichia coli strain BL21 ung151 (ung::Tn10(Tet)) | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an ung-1 mutant has reduced ability to repair uracil-containing DNA. Ung-1 mutants show altered levels of apoptotic cell corpses formed in response to DNA damaging agents and increased apoptosis in response to ionizing radiation. The phenotype is a consequence of compensatory transcriptomic shifts that modulate oxidative stress responses in the mutant and not an effect of reduced DNA damage signaling, overview. Attenuation of paraquat-induced apoptosis in ung-1 mutant results from modulation of stress-induced signaling pathways, overview | Caenorhabditis elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ugi | a peptide inhibitor specific for UNG-family enzyme | Caenorhabditis elegans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 60000, recombinant N-terminally GST-tagged enzyme, SDS-PAGE, x * 32000, detagged enzyme, SDS-PAGE | Caenorhabditis elegans |
60000 | - |
x * 60000, recombinant N-terminally GST-tagged enzyme, SDS-PAGE, x * 32000, detagged enzyme, SDS-PAGE | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
gene ung-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally GST-tagged enzyme from Escherichia coli strain BL21 ung151 (ung::Tn10(Tet)) by glutathione affinity chromatography, cleavage of the tag by thrombin and further purification by cationic exchange chromatography | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows activity on double-stranded as well as single-stranded DNAs, the assay uses a 19mer oligodeoxyribonucleotide substrate containing a single uracil residue (5'-CATAAAGTGUAAAGCCTGG-3') and a guanine or adenine residue opposite uracil | Caenorhabditis elegans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant N-terminally GST-tagged enzyme, SDS-PAGE, x * 32000, detagged enzyme, SDS-PAGE | Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the UNG family of enzymes | Caenorhabditis elegans |
UDG | - |
Caenorhabditis elegans |
UNG-1 | - |
Caenorhabditis elegans |
uracil-DNA glycosylase | - |
Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Caenorhabditis elegans |
General Information | Comment | Organism |
---|---|---|
physiological function | UNG-1 contributes to repair of ionizing radiation-induced DNA base damage in vivo | Caenorhabditis elegans |