Cloned (Comment) | Organism |
---|---|
vcUNG, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Vibrio cholerae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, hanging drop vapour diffusion, mixing of 0.001 ml of protein solution containing 15 mg/ml protein in 50 mM Tris-HCl, pH 7.5, and 5 mM 2-mercaptoethanol, with 0.001 ml of reservoir solution containing 50 mM Na MES, pH 6.0, and 28% w/v PEG 3350, equilibration against a reservoir containing 1.0 ml precipitant solution, room temperature, 1 week, X-ray diffraction structure determination and analysis at 1.5 A resolution, modelling | Vibrio cholerae |
Protein Variants | Comment | Organism |
---|---|---|
H194R | site-directed mutagenesis, the mutant shows altered kinetics compared to the wild-type enzyme | Vibrio cholerae |
V90R | site-directed mutagenesis, the mutant shows altered kinetics compared to the wild-type enzyme | Vibrio cholerae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
dUMP DNA | pH 7.5, recombinant wild-type enzyme | Aliivibrio salmonicida | |
0.0011 | - |
dUMP DNA | pH 7.5, recombinant mutant V90R | Vibrio cholerae | |
0.0018 | - |
dUMP DNA | pH 7.5, recombinant mutant H194R | Vibrio cholerae | |
0.0028 | - |
dUMP DNA | pH 7.5, recombinant wild-type enzyme | Vibrio cholerae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio salmonicida | - |
- |
- |
Vibrio cholerae | Q9KPK8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Vibrio cholerae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUMP DNA + H2O | - |
Vibrio cholerae | dUMP + DNA | - |
? | |
dUMP DNA + H2O | - |
Aliivibrio salmonicida | dUMP + DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | asUNG structure homology modelling based on the crystal structure of vcUNG from Vibrio cholerae, asUNG possesses typical cold-adapted features compared with vcUNG, overview | Aliivibrio salmonicida |
More | UNG structure modelling, overview | Vibrio cholerae |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the uracil DNA glycosylase family | Vibrio cholerae |
More | the enzyme belongs to the uracil DNA glycosylase family | Aliivibrio salmonicida |
UNG | - |
Vibrio cholerae |
UNG | - |
Aliivibrio salmonicida |
uracil-DNA N-glycosylase | - |
Vibrio cholerae |
uracil-DNA N-glycosylase | - |
Aliivibrio salmonicida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
154 | - |
dUMP DNA | pH 7.5, recombinant mutant V90R | Vibrio cholerae | |
174 | - |
dUMP DNA | pH 7.5, recombinant wild-type enzyme | Aliivibrio salmonicida | |
293 | - |
dUMP DNA | pH 7.5, recombinant wild-type enzyme | Vibrio cholerae | |
821 | - |
dUMP DNA | pH 7.5, recombinant mutant H194R | Vibrio cholerae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Vibrio cholerae |
7.5 | - |
assay at | Aliivibrio salmonicida |