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Literature summary for 3.2.2.27 extracted from

  • Nakamura, N.; Morinaga, H.; Kikuchi, M.; Yonekura, S.-I.; Ishii1, N.; Yamamoto, K.; Yonei S.; Zhang, Q.-M.
    Cloning and characterization of uracil-DNA glycosylase and the biological consequences of the loss of its function in the nematode Caenorhabditis elegans (2008), Mutagenesis, 23, 407-413.
    View publication on PubMed
Search for more literature for 3.2.2.27

Cloned(Commentary)

Cloned (Comment) Organism
gene ung-1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
additional information a Ung-1 defective mutant strain TM2862 shows no uracil excision activity, but the mutation in the ung-1 gene does not affect development, fertility and lifespan in Caenorhanditis elegans, suggesting the existence of backup enzyme Caenorhabditis elegans

Inhibitors

Inhibitors Comment Organism Structure
Bacillus subtilis Ung inhibitor
-
 Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, about, sequence calculation Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O Caenorhabditis elegans
-
 uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O Caenorhabditis elegans Bristol N2
-
 uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched single-stranded DNA + H2O Caenorhabditis elegans
-
 uracil + single-stranded DNA with abasic site
-
 ?
uracil-mismatched single-stranded DNA + H2O Caenorhabditis elegans Bristol N2
-
 uracil + single-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-
Caenorhabditis elegans Bristol N2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Ung-1 from Escherichia coli strain BL21 Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UNG-1 removes uracil from both U-G and U-A base pairs in DNA. It also removes uracil from single-stranded oligonucleotide substrate less efficiently than double-stranded oligonucleotide. The active site A is present between residues 116-137, active site B at residues 247-253. Aromatic residues Tyr125, Phe136 and His247 are involved in the stacking interaction with uracil Caenorhabditis elegans ?
-
 ?
additional information UNG-1 removes uracil from both U-G and U-A base pairs in DNA. It also removes uracil from single-stranded oligonucleotide substrate less efficiently than double-stranded oligonucleotide. The active site A is present between residues 116-137, active site B at residues 247-253. Aromatic residues Tyr125, Phe136 and His247 are involved in the stacking interaction with uracil Caenorhabditis elegans Bristol N2 ?
-
 ?
uracil-mismatched double-stranded DNA + H2O
-
 Caenorhabditis elegans uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O
-
 Caenorhabditis elegans Bristol N2 uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched single-stranded DNA + H2O
-
 Caenorhabditis elegans uracil + single-stranded DNA with abasic site
-
 ?
uracil-mismatched single-stranded DNA + H2O
-
 Caenorhabditis elegans Bristol N2 uracil + single-stranded DNA with abasic site
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, about, sequence calculation Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
More UNG-1 belongs to the UNG family 1 Caenorhabditis elegans
UNG-1
-
 Caenorhabditis elegans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
 assay at Caenorhabditis elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Caenorhabditis elegans