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Literature summary for 3.2.2.27 extracted from
- hUNG2 is the major repair enzyme for removal of uracil from U:A matches, U:G mismatches, and U in single-stranded DNA, with hSMUG1 as a broad specificity backup (2002), J. Biol. Chem., 277, 39926-39936.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| APE1 | has a strong stimulatory effect on hSMUG1 against dsU, apparently because of enhanced dissociation of hSMUG1 from AP sites in dsDNA. AP sites inhibit hSMUG1 | Homo sapiens | |
| additional information | AP sites do not inhibit hUNG2 | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of hSMUG1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
| expression of hUNG2 in Escherichia coli strain BL21 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | UNG2 kinetics | Homo sapiens | |
| 0.0004 | - |
uracil-mismatched double-stranded DNA with U-G mismatch | pH 7.5, 30°C, recombinant hUNG2 | Homo sapiens | |
| 0.0007 | - |
uracil-mismatched double-stranded DNA with U-A mismatch | pH 7.5, 30°C, recombinant hUNG2 | Homo sapiens | |
| 0.0013 | - |
uracil-mismatched double-stranded DNA with U-G mismatch | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 0.0017 | - |
uracil-mismatched single-stranded DNA | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 0.0022 | - |
uracil-mismatched single-stranded DNA | pH 7.5, 30°C, recombinant hUNG2 | Homo sapiens | |
| 0.0027 | - |
5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 0.0038 | - |
5-hydroxymethyluracil-mismatched single-stranded DNA | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 0.004 | - |
uracil-mismatched double-stranded DNA with U-A mismatch | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 0.0046 | - |
5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleolus | hSMUG1 accumulates in nucleoli in living cells | Homo sapiens | 5730 | - |
| nucleolus | hUNG2 is excluded from nucleoli in living cells, but hUNG2 accumulates in replication foci in the S-phase | Homo sapiens | 5730 | - |
| nucleus | hUNG2 | Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strongly stimulating at physiological concentrations | Homo sapiens |  |
| Mg2+ | strongly stimulating at physiological concentrations, 10fold for Ung2 at 10 mM. Mg2+ increases the preference of hUNG2 toward uracil in ssDNA nearly 40fold | Homo sapiens | |
| NaCl | hSMUG1 is stimulated by 30% at 70 mM NaCl | Homo sapiens | |
| NaCl | hUNG2 is stimulated 2fold by 50 mM NaCl | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,N4-ethenocytosine-mismatched single-stranded DNA + H2O | Homo sapiens | - |
3,N4-ethenocytosine + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxyuracil-mismatched single-stranded DNA + H2O | Homo sapiens | - |
5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
| additional information | Homo sapiens | hSMUG1 removes uracil from both double- and single-stranded DNA in nuclear chromatin, hSMUG1 has a broad substrate specificity, including 5-hydroxymethyluracil, and 3,N4-ethenocytosine. hSMUG1 acts as a broad specificity backup and is the major 5-hydroxymethyluracil-DNA glycosylase in nuclear cell extracts, overview | ? | - |
? | |
| additional information | Homo sapiens | hUNG2 removes uracil from both double- and single-stranded DNA in nuclear chromatin. hUNG2 in nuclear extracts initiates base excision repair of plasmids containing either U-A and U-G in vitro. hUNG2 is responsible for both prereplicative removal of deaminated cytosine and postreplicative removal of misincorporated uracil at the replication fork, it is the major enzyme for removal of deaminated cytosine outside of replication foci, overview | ? | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched single-stranded DNA + H2O | Homo sapiens | - |
uracil + single-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | P22674 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant hSMUG1 from Escherichia coli strain BL21(DE3) by protamine sulfate precipitation, hydrophobic interaction and adsorption chromatography, ultrafiltration, ion exchange chromatography, polyU-affinity, and again ion exchange chromatography. Recombinant hUNG2 from Escherichia coli strain BL21 by gel filtration, ion exchange chromatography, and ultrafiltration | Homo sapiens |
| recombinant hUNG2 from Escherichia coli strain BL21 by gel filtration, ion exchange chromatography, and ultrafiltration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| colorectal carcinoma cell | - |
Homo sapiens | - |
| CX-1 cell | - |
Homo sapiens | - |
| HaCaT cell | - |
Homo sapiens | - |
| HeLa cell | - |
Homo sapiens | - |
| keratinocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
catalytic efficiencies of hUNG2 and hSMUG1 | Homo sapiens |
| additional information | - |
catalytic efficiency of hSMUG1 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,N4-ethenocytosine-mismatched single-stranded DNA + H2O | - |
Homo sapiens | 3,N4-ethenocytosine + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch + H2O | - |
Homo sapiens | 5-hydroxymethyluracil + double-stranded DNA with abasic site | - |
? | |
| 5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch + H2O | - |
Homo sapiens | 5-hydroxymethyluracil + double-stranded DNA with abasic site | - |
? | |
| 5-hydroxymethyluracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxymethyluracil + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxyuracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
| additional information | substrate specificity, overview | Homo sapiens | ? | - |
? | |
| additional information | hSMUG1 removes uracil from both double- and single-stranded DNA in nuclear chromatin, hSMUG1 has a broad substrate specificity, including 5-hydroxymethyluracil, and 3,N4-ethenocytosine. hSMUG1 acts as a broad specificity backup and is the major 5-hydroxymethyluracil-DNA glycosylase in nuclear cell extracts, overview | Homo sapiens | ? | - |
? | |
| additional information | hUNG2 removes uracil from both double- and single-stranded DNA in nuclear chromatin. hUNG2 in nuclear extracts initiates base excision repair of plasmids containing either U-A and U-G in vitro. hUNG2 is responsible for both prereplicative removal of deaminated cytosine and postreplicative removal of misincorporated uracil at the replication fork, it is the major enzyme for removal of deaminated cytosine outside of replication foci, overview | Homo sapiens | ? | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA with U-A mismatch + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA with U-G mismatch + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | uracil + single-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| single-strand selective monofunctional uracil-DNA glycosylase | - |
Homo sapiens |
| SMUG1 | - |
Homo sapiens |
| UNG2 | - |
Homo sapiens |
| uracil-DNA glycosylase 2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 0.04 | - |
5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 0.052 | - |
uracil-mismatched double-stranded DNA with U-A mismatch | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 0.055 | - |
uracil-mismatched double-stranded DNA with U-G mismatch | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 0.098 | - |
5-hydroxymethyluracil-mismatched single-stranded DNA | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 0.29 | - |
uracil-mismatched single-stranded DNA | pH 7.5, 30°C, recombinant hSMUG1 | Homo sapiens | |
| 2.28 | - |
uracil-mismatched double-stranded DNA with U-A mismatch | pH 7.5, 30°C, recombinant hUNG2 | Homo sapiens | |
| 5.57 | - |
uracil-mismatched double-stranded DNA with U-G mismatch | pH 7.5, 30°C, recombinant hUNG2 | Homo sapiens | |
| 46.1 | - |
uracil-mismatched single-stranded DNA | pH 7.5, 30°C, recombinant hUNG2 | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | hSMUG1 | Homo sapiens |
| 7 | 7.5 | hUNG2 | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH profile of hSMUG1 | Homo sapiens |
| additional information | - |
pH profile of hUNG2 | Homo sapiens |
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