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Literature summary for 3.2.2.27 extracted from
- Uracil-DNA glycosylase in the extreme thermophile Archaeoglobus fulgidus (2000), J. Biol. Chem., 275, 19146-19149.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Archaeoglobus fulgidus |
| gene ung, expression of His-tagged enzyme in Escherichia coli strain BW310(DE3) | Archaeoglobus fulgidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| apurinic/apyrimidinic site | the enzyme is product-inhibited by both uracil and apurinic/apyrimidinic sites | Archaeoglobus fulgidus | |
| additional information | UDG is product-inhibited by both uracil and apurinic/apyrimidinic sites | Archaeoglobus fulgidus | |
| Uracil | the enzyme is product-inhibited by both uracil and apurinic/apyrimidinic sites; UDG is product-inhibited by both uracil and apurinic/apyrimidinic sites | Archaeoglobus fulgidus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C | Archaeoglobus fulgidus | |
| 0.0005 | - |
uracil-mismatched single-stranded DNA | pH 7.5, 37°C | Archaeoglobus fulgidus | |
| 0.0005 | - |
uracil-mismatched double-stranded DNA | pH 7.8, 70°C | Archaeoglobus fulgidus | |
| 0.0005 | - |
uracil-mismatched single-stranded DNA | pH 7.8, 95°C | Archaeoglobus fulgidus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded DNA + H2O | Archaeoglobus fulgidus | - |
uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched single-stranded DNA + H2O | Archaeoglobus fulgidus | - |
uracil + single-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
gene ung | - |
| Archaeoglobus fulgidus | O28007 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Archaeoglobus fulgidus |
| recombinant His-tagged enzyme from Escherichia coli strain BW310(DE3) by nickel affinity and anion exchange chromatography | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded DNA + H2O | - |
Archaeoglobus fulgidus | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | removing uracil from double-stranded DNA containing either a U-A or U-G base pair | Archaeoglobus fulgidus | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | is capable of removing uracil from double-stranded DNA containing either a U/A or U/G base pair | Archaeoglobus fulgidus | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched single-stranded DNA + H2O | - |
Archaeoglobus fulgidus | uracil + single-stranded DNA with abasic site | - |
? | |
| uracil-mismatched single-stranded DNA + H2O | removing uracil from single-stranded DNA containing either a U-A or U-G base pair | Archaeoglobus fulgidus | uracil + single-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AFUDG | - |
Archaeoglobus fulgidus |
| UDG | - |
Archaeoglobus fulgidus |
| UNG | - |
Archaeoglobus fulgidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
1.5 h, completely stable with no loss of activity | Archaeoglobus fulgidus |
| 95 | - |
1.5 h, enzyme maintains full activity | Archaeoglobus fulgidus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.55 | - |
uracil-mismatched double-stranded DNA | pH 7.5, 37°C | Archaeoglobus fulgidus | |
| 0.55 | - |
uracil-mismatched single-stranded DNA | pH 7.8, 95°C | Archaeoglobus fulgidus | |
| 0.917 | - |
uracil-mismatched single-stranded DNA | pH 7.5, 37°C | Archaeoglobus fulgidus | |
| 0.92 | - |
uracil-mismatched double-stranded DNA | pH 7.8, 70°C | Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | - |
Archaeoglobus fulgidus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is essential for maintaining genomic integrity | Archaeoglobus fulgidus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1100 | - |
uracil-mismatched single-stranded DNA | pH 7.8, 95°C | Archaeoglobus fulgidus | |
| 1840 | - |
uracil-mismatched double-stranded DNA | pH 7.8, 70°C | Archaeoglobus fulgidus |
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