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Literature summary for 3.2.2.27 extracted from
- Characterization of an archaeal family 4 uracil DNA glycosylase and its interaction with PCNA and chromatin proteins (2005), Biochem. J., 387, 859-863.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation of UDG1 by proliferating cell nuclear antigen, PCNA | Saccharolobus solfataricus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-tagged UDG1 in Escherichia coli, expression of UDG1 with PCNA in the yeast two-hybrid system using Saccharomyces cerevisiae strain AH109 | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Alba protein | a small basic chromatin protein, repression of UDG1 activity | Saccharolobus solfataricus | |
| Sso7d protein | a small basic chromatin protein, slight repression of UDG1 activity | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded DNA + H2O | Saccharolobus solfataricus | the formation of archaeal chromatin is highly repressive to UDG1 activity, mechanistic basis for coupling UDG1 to the replication fork, modelling, overview | uracil + double-stranded DNA with abasic site | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
gene udg1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimal growth at 80°C | Saccharolobus solfataricus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded DNA + H2O | the formation of archaeal chromatin is highly repressive to UDG1 activity, mechanistic basis for coupling UDG1 to the replication fork, modelling, overview | Saccharolobus solfataricus | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | UDG1 shows a marked preference for substrates containing a G-U base pair over either A-U or single-stranded uracil-containing DNA substrates, and it interacts with a single subunit of the heterotrimeric sliding clamp proliferating cell nuclear antigen, PCNA, in a PIP motif-dependent manner, a conserved C-terminal consensus interaction motif, overview | Saccharolobus solfataricus | uracil + double-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the archaeal family 4 | Saccharolobus solfataricus |
| UDG1 | - |
Saccharolobus solfataricus |
| uracil DNA glycosylase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
recombinant enzyme | Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 99 | - |
Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharolobus solfataricus |
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