Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.23 extracted from

  • Reddy, P.T.; Jaruga, P.; Nelson, B.C.; Lowenthal, M.; Dizdaroglu, M.
    Stable isotope-labeling of DNA repair proteins, and their purification and characterization (2011), Protein Expr. Purif., 78, 94-101.
    View publication on PubMed
Search for more literature for 3.2.2.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

General Stability

General Stability Organism
15N-labeled formamidopyrimidine DNA glycosylase is as active as unlabeled enzyme Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Escherichia coli
30290
-
 x * 30290, calculated from amino acid sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DE52 resin column chromatography and HPLC-Shodex carboxymethyl cellulose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
-
 Escherichia coli 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
 ?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
-
 Escherichia coli 4,6-diamino-5-formamidopyrimidine + DNA
-
 ?
DNA containing 8-hydroxyguanine residues + H2O
-
 Escherichia coli 8-hydroxyguanine + DNA
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Escherichia coli
? x * 30290, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
formamidopyrimidine DNA glycosylase
-
 Escherichia coli
FPG
-
 Escherichia coli
MutM
-
 Escherichia coli