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Literature summary for 3.2.2.23 extracted from
- Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase (2009), FEBS J., 276, 5149-5162.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the human AP endonuclease APEX1 can stimulate wild-type OGG1 activity by increasing its turnover rate, but very little stimulation of 8-oxoGua removal in the presence of APEX1 occurs with phosphomimetic mutants, overview | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genotyping, overview. Expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A288V | naturally occuring polymorphism, the mutant displays opposite-base specificity similar to that of wild-type OGG1, activity, substrate specificity and kinetics compared to the wild-type enzyme, overview | Homo sapiens |
| D322N | naturally occuring polymorphism, the mutant is 2.3fold more specific for the correct opposite base than the wild-type enzyme, activity, substrate specificity and kinetics compared to the wild-type enzyme | Homo sapiens |
| additional information | the gene shows several polymorphisms in vivo | Homo sapiens |
| S231E | naturally occuring polymorphism, kinetics compared to the wild-type enzyme, overview | Homo sapiens |
| S231E/S232E | naturally occuring polymorphism, kinetics compared to the wild-type enzyme, overview | Homo sapiens |
| S232E | naturally occuring polymorphism, kinetics compared to the wild-type enzyme, overview | Homo sapiens |
| S280E | naturally occuring polymorphism, kinetics compared to the wild-type enzyme, overview | Homo sapiens |
| S326C | naturally occuring polymorphism, the mutant displays opposite-base specificity similar to that of wild-type OGG1. The mutant efficiently excises 8-oxoGua from oligodeoxynucleotides and 2,6-diamino-4-hydroxy-5-formamidopyrimidine from gamma-irradiated DNA, but excises 8-oxoG rather inefficiently from gamma-irradiated DNA | Homo sapiens |
| S326E | naturally occuring polymorphism, kinetics compared to the wild-type enzyme, overview | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000034 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S326C | Homo sapiens | |
| 0.0000034 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, wild-type enzyme | Homo sapiens | |
| 0.0000057 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S231E | Homo sapiens | |
| 0.0000061 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant D322N | Homo sapiens | |
| 0.0000074 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S280E | Homo sapiens | |
| 0.0000075 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S326E | Homo sapiens | |
| 0.0000086 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant A288V | Homo sapiens | |
| 0.0000092 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S232E | Homo sapiens | |
| 0.00001 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S231E/S232E | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt-DNA + H2O | Homo sapiens | - |
2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt-DNA | - |
? |  |
| 8-oxo-7,8-dihydroguanine:Cyt-DNA + H2O | Homo sapiens | - |
8-oxo-7,8-dihydroguanine + Cyt-DNA | - |
? | |
| additional information | Homo sapiens | 8-oxo-7,8-dihydroguanine, i.e. 8-oxoGua, and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, i.e. FapyGua, are premutagenic DNA lesions that appear in DNA damaged by reactive oxygen species of endogenous and environmental origin, and are excised from DNA by the enzyme. The fidelity of the 8-oxoGua repair system depends on discrimination between 8-oxoGua:Cyt and 8-oxoGua:Ade pairs by OGG1 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O15527 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylation of OGG1 can affect its biological functions at several levels, including the intrinsic activity and intracellular localization | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity of wild-type and mutant enzymes, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt oligodeoxynucleotide + H2O | - |
Homo sapiens | 2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt oligodeoxynucleotide | - |
? | |
| 2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt-DNA + H2O | - |
Homo sapiens | 2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt-DNA | - |
? | |
| 8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide + H2O | - |
Homo sapiens | 8-oxo-7,8-dihydroguanine + Cyt oligodeoxynucleotide | - |
? | |
| 8-oxo-7,8-dihydroguanine:Cyt-DNA + H2O | - |
Homo sapiens | 8-oxo-7,8-dihydroguanine + Cyt-DNA | - |
? | |
| additional information | 8-oxo-7,8-dihydroguanine, i.e. 8-oxoGua, and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, i.e. FapyGua, are premutagenic DNA lesions that appear in DNA damaged by reactive oxygen species of endogenous and environmental origin, and are excised from DNA by the enzyme. The fidelity of the 8-oxoGua repair system depends on discrimination between 8-oxoGua:Cyt and 8-oxoGua:Ade pairs by OGG1 | Homo sapiens | ? | - |
? | |
| additional information | 8-oxoguanine-DNA glycosylase, OGG1, efficiently removes mutagenic 8-oxo-7,8-dihydroguanine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine when paired with cytosine in oxidatively damaged DNA. Excision of 8-oxoGua mispaired with adenine may lead to G to T transversions. Substrate specificity of wild-type and mutant enzymes, overview | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | three-dimensional structure of OGG1, PDB ID 1EBM, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 8-oxoguanine-DNA glycosylase | - |
Homo sapiens |
| OGG1 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00037 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S326C | Homo sapiens | |
| 0.00047 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant D322N | Homo sapiens | |
| 0.00048 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S280E | Homo sapiens | |
| 0.0005 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, wild-type enzyme | Homo sapiens | |
| 0.00053 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S326E | Homo sapiens | |
| 0.00065 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S232E | Homo sapiens | |
| 0.00068 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S231E/S232E | Homo sapiens | |
| 0.0007 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant S231E | Homo sapiens | |
| 0.00092 | - |
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide | pH 7.4-7.5, mutant A288V | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.5 | assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | OGG1 polymorphisms might be associated with succeptibility of humans to cancer and other diseases | Homo sapiens |
| physiological function | OGG1 is part of an enzymatic system responsible for prevention of mutations generated by 8-oxo-7,8-dihydroguanine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine | Homo sapiens |
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