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Literature summary for 3.2.2.23 extracted from
- Heavy metal ions affect the activity of DNA glycosylases of the fpg family (2009), Biochemistry (Moscow), 74, 1253-1259.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
Homo sapiens |  |
| Cd2+ | preferentially binds to DNA bases rather than phosphates, the presence of the metal ions causes the enzyme to lose the ability for preferential binding to damaged DNA | Homo sapiens | |
| Co2+ | - |
Homo sapiens | |
| Cu2+ | preferentially binds to DNA bases rather than phosphates, the presence of the metal ions causes the enzyme to lose the ability for preferential binding to damaged DNA | Homo sapiens | |
| Fe2+ | - |
Homo sapiens | |
| additional information | differing inhibitory effects of metal ions in potassiumphosphate buffer and Tris-HCl buffer, mechanism of NEIL1 inhibition by heavy metal salts, e.g. involving sequential elimination of phosphate groups from the 3'- and 5'-side of the formed AP site, overview | Homo sapiens | |
| Ni2+ | - |
Homo sapiens | |
| Zn2+ | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady state kinetics | Homo sapiens | |
| 0.00015 | - |
5,6-dihydrouracil-DNA | NEIL1 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | NEIL1 is not affected by Mg2+, Mn2+, and Ca2+ | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil-DNA + H2O | Homo sapiens | DHU is excised from DNA by a number of DNA glycosylases including Fpg and Nei | 5,6-dihydrouracil + DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil-DNA + H2O | DHU is excised from DNA by a number of DNA glycosylases including Fpg and Nei | Homo sapiens | 5,6-dihydrouracil + DNA | - |
? | |
| 5,6-dihydrouracil-DNA + H2O | DHU is formed in DNA from cytosine under the action of OH radicals under ionizing radiation | Homo sapiens | 5,6-dihydrouracil + DNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| formamidopyrimidineDNA glycosylase | - |
Homo sapiens |
| FPG | - |
Homo sapiens |
| More | the enzymes belong to the Fpg family of DNA glycosylases | Homo sapiens |
| Nei | - |
Homo sapiens |
| NEIL1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
5,6-dihydrouracil-DNA | NEIL1 | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
- |
Homo sapiens | Cd2+ | |
| 0.016 | - |
- |
Homo sapiens | Zn2+ | |
| 0.4 | - |
- |
Homo sapiens | Cu2+ | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme initiates the process of repair of oxidized DNA bases | Homo sapiens |
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