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Literature summary for 3.2.2.23 extracted from

  • Minetti, C.A.; Remeta, D.P.; Breslauer, K.J.
    A continuous hyperchromicity assay to characterize the kinetics and thermodynamics of DNA lesion recognition and base excision (2008), Proc. Natl. Acad. Sci. USA, 105, 70-75.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000181
-
 13mer oligonucleotide duplex containing 8-oxoguanine 15°C Escherichia coli
0.0000222
-
 13mer oligonucleotide duplex containing 8-oxoguanine 17.5°C Escherichia coli
0.0000296
-
 13mer oligonucleotide duplex containing 8-oxoguanine 20°C Escherichia coli
0.0000369
-
 13mer oligonucleotide duplex containing 8-oxoguanine 22.5°C Escherichia coli
0.0000466
-
 13mer oligonucleotide duplex containing 8-oxoguanine 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine formation of the transition state complex from the reactants exhibits an overall free energy of 9.6 kcal/mol Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
13mer oligonucleotide duplex containing 8-oxoguanine + H2O
-
 Escherichia coli 8-oxoguanine + oligonucleotide
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
 13mer oligonucleotide duplex containing 8-oxoguanine 15°C Escherichia coli
0.0106
-
 13mer oligonucleotide duplex containing 8-oxoguanine 17.5°C Escherichia coli
0.0148
-
 13mer oligonucleotide duplex containing 8-oxoguanine 20°C Escherichia coli
0.0202
-
 13mer oligonucleotide duplex containing 8-oxoguanine 22.5°C Escherichia coli
0.0273
-
 13mer oligonucleotide duplex containing 8-oxoguanine 25°C Escherichia coli