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Literature summary for 3.2.2.23 extracted from

  • Fedorova, O.S.; Nevinsky, G.A.; Koval, V.V.; Ishchenko, A.A.; Vasilenko, N.L.; Douglas, K.T.
    Stopped-flow kinetic studies of the interaction between Escherichia coli Fpg protein and DNA substrates (2002), Biochemistry, 41, 1520-1528.
    View publication on PubMed
Search for more literature for 3.2.2.23

Inhibitors

Inhibitors Comment Organism Structure
d(pA)10-d(pT)10 dublex, nonspecific oligonucleotide Escherichia coli
d(pA)16-d(pT)16 dublex, nonspecific oligonucleotide Escherichia coli
nonspecific ds 8-23mer oligonucleotide competitive inhibitors Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 DNA containing 7-hydro-8-oxoguanine residues pH 7.5, 25°C Escherichia coli
0.0046
-
 DNA pH 7.5, 25°C, DNA containing an apurinic/apyrimidinic site Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Escherichia coli removes oxidized purines from oxidatively damaged DNA ?
-
 ?
DNA + H2O Escherichia coli DNA base excision repair enzyme ?
-
 ?
DNA containing 7-hydro-8-oxoguanine + H2O Escherichia coli repairs oxidative DNA damage by efficiently removing formamidopyrimidine lesions and 8-oxoguanine residues from DNA 7-hydro-8-oxoguanine + DNA
-
 ?
DNA containing 7-hydro-8-oxoguanine + H2O Escherichia coli dublex, primary physiological substrate, DNA repair 7-hydro-8-oxoguanine + DNA
-
 ?
DNA containing ring-opened N7-methylguanine + H2O Escherichia coli repairs oxidative DNA damage by efficiently removing formamidopyrimidine lesions and 8-oxoguanine residues from DNA 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine kinetic mechanism Escherichia coli
a
DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine catalytic mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions Escherichia coli ?
-
 ?
DNA + H2O removes oxidized purines from oxidatively damaged DNA Escherichia coli ?
-
 ?
DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Escherichia coli ?
-
 ?
DNA + H2O DNA base excision repair enzyme Escherichia coli ?
-
 ?
DNA containing 7-hydro-8-oxoguanine + H2O repairs oxidative DNA damage by efficiently removing formamidopyrimidine lesions and 8-oxoguanine residues from DNA Escherichia coli 7-hydro-8-oxoguanine + DNA
-
 ?
DNA containing 7-hydro-8-oxoguanine + H2O dublex, primary physiological substrate, DNA repair Escherichia coli 7-hydro-8-oxoguanine + DNA
-
 ?
DNA containing 7-hydro-8-oxoguanine residues + H2O kinetic mechanism, 3 activities: DNA-glycosylase, beta-elimination/AP-lyase and delta elimination, 12-nucleotide dublex containing 8-oxo-G in the sixth position of one strand, conformational transitions of Fpg protein during the catalytic process Escherichia coli DNA + 7-hydro-8-oxoguanine
-
 ?
DNA containing ring-opened N7-methylguanine + H2O repairs oxidative DNA damage by efficiently removing formamidopyrimidine lesions and 8-oxoguanine residues from DNA Escherichia coli 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
 DNA containing 7-hydro-8-oxoguanine residues pH 7.5, 25°C Escherichia coli
0.05
-
 DNA pH 7.5, 25°C, DNA containing an apurininc/apyrimidinic site Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information with increase in dublex length of nonspecific ds 8-23mer oligonucleotides, Ki values decrease from 0.05 mM to 0.0007 mM Escherichia coli
0.001
-
 d(pA)16-d(pT)16 pH 7.5, 25°C Escherichia coli
0.0067
-
 d(pA)10-d(pT)10 pH 7.5, 25°C Escherichia coli