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Literature summary for 3.2.2.23 extracted from

  • Sidorkina, O.; Dizdaroglu, M.; Laval, J.
    Effect of single mutations on the specificity of Escherichia coli FPG protein for excision of purine lesions from DNA damaged by free radicals (2001), Free Radic. Biol. Med., 31, 816-823.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
K155A effect of mutation on specificity, mutant with very low activity, kinetic study Escherichia coli
K57G effect of mutation on specificity, mutant removes FapyAde and FapyGua with reduced activity compared to wild-type Fpg, kinetic study Escherichia coli
K57R effect of mutation on specificity, mutant removes FapyAde and FapyGua with reduced activity compared to wild-type Fpg, kinetic study Escherichia coli
P2E effect of mutation on specificity, inactive mutant, kinetic study Escherichia coli
P2G effect of mutation on specificity, mutant with very low activity, kinetic study Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00078 0.00084 DNA containing 4,6-diamino-5-formamidopyrimidine residues pH 7.4, 37°C, K57G mutant Fpg Escherichia coli
0.00079 0.0009 DNA containing 4,6-diamino-5-formamidopyrimidine residues pH 7.4, 37°C, K57R mutant Fpg Escherichia coli
0.00093 0.00155 DNA containing 8-hydroxyguanine residues pH 7.4, 37°C, K57R mutant Fpg Escherichia coli
0.00107 0.00129 DNA containing 4,6-diamino-5-formamidopyrimidine residues pH 7.4, 37°C, wild-type Fpg Escherichia coli
0.00109
-
DNA containing 8-hydroxyguanine residues pH 7.4, 37°C, wild-type Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc Escherichia coli
0.00126
-
DNA containing 8-hydroxyguanine residues pH 7.4, 37°C, K57G mutant Fpg Escherichia coli
0.00178
-
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues pH 7.4, 37°C, K57R mutant Fpg, from DNA gamma-irradiated under NO2 Escherichia coli
0.00187
-
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues pH 7.4, 37°C, K57G mutant Fpg, from DNA gamma-irradiated under NO2 Escherichia coli
0.00311
-
DNA containing 8-hydroxyguanine residues pH 7.4, 37°C, wild-type Fpg, from DNA gamma-irradiated under NO2 Escherichia coli
0.00343 0.00376 DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues pH 7.4, 37°C, wild-type Fpg Escherichia coli
0.00422
-
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues pH 7.4, 37°C, K57G mutant Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc Escherichia coli
0.00487
-
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues pH 7.4, 37°C, K57R mutant Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains a four cystein-zinc finger motif Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Escherichia coli DNA repair enzyme specific for the removal of purine-derived lesions from DNA damaged by free radicals and other oxidative processes ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
K57R, K155A mutant Fpg Escherichia coli
wild-type and K57G, P2G, P2E mutant Fpg Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O removes oxidized purines from oxidatively damaged DNA Escherichia coli ?
-
?
DNA + H2O active site is located within the first 73 amino acids of the N-terminus Escherichia coli ?
-
?
DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Escherichia coli ?
-
?
DNA + H2O DNA repair enzyme specific for the removal of purine-derived lesions from DNA damaged by free radicals and other oxidative processes Escherichia coli ?
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O removal with similar specificity as 4,6-diamino-5-formamidopyrimidine and 8-hydroxyguanine Escherichia coli 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O FapyGua Escherichia coli 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O removal with similar specificity as 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-hydroxyguanine Escherichia coli 4,6-diamino-5-formamidopyrimidine + DNA
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O FapyAde Escherichia coli 4,6-diamino-5-formamidopyrimidine + DNA
-
?
DNA containing 8-hydroxyadenine residues + H2O poor substrate Escherichia coli 8-hydroxyadenine + DNA
-
?
DNA containing 8-hydroxyguanine residues + H2O removal with similar specificity as 4,6-diamino-5-formamidopyrimidine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, important role of Lys-57 in Fpg activity for 8-hydroxyguanine, but lesser significant role for formamidopyrimidines, Pro-2 is critical for substrate recognition and in catalysis of its excision Escherichia coli 8-hydroxyguanine + DNA
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
-
Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Escherichia coli