Literature summary for 3.2.2.22 extracted from

Di, R.; Kyu, E.; Shete, V.; Saidasan, H.; Kahn, P.C.; Tumer, N.E.
Identification of amino acids critical for the cytotoxicity of Shiga toxin 1 and 2 in Saccharomyces cerevisiae (2011), Toxicon, 57, 525-539.

Search for more literature for 3.2.2.22

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae strain W303	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
E167K	the mutation reduces the toxicity of Shiga toxins 1 and 2	Escherichia coli
E167K/R176K	the mutations reduce the toxicity of Shiga toxins 1 and 2	Escherichia coli
N75A	the mutation reduces the toxicity of Shiga toxins 1 and 2	Escherichia coli
R170A	ribosomes are depurinated at 53% and 65% of the wild type in yeast expressing Stx1A-R170A and Stx2A-R170A, respectively	Escherichia coli
R170A	the mutation reduces the toxicity of Shiga toxins 1 and 2	Escherichia coli
R176K	the mutation reduces the toxicity of Shiga toxins 1 and 2	Escherichia coli
Y77A	the mutation reduces the toxicity of Shiga toxins 1 and 2	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Escherichia coli	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32500	-	x * 32500, mature enzyme, SDS-PAGE	Escherichia coli
35000	-	x * 35000, precursor, SDS-PAGE	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	ATCC43895	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
25S rRNA + H2O	depurination	Escherichia coli	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 32500, mature enzyme, SDS-PAGE	Escherichia coli
?	x * 35000, precursor, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
ribosome inactivating protein	-	Escherichia coli
RIP	-	Escherichia coli
Shiga toxin 1 subunit A	-	Escherichia coli
Shiga toxin 2 subunit A	-	Escherichia coli
Stx1A	-	Escherichia coli
Stx2A	-	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	the enzyme inhibits translation by removing an adenine from the highly conserved alha-sarcin ricin loop of the large rRNA	Escherichia coli

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Release 2023.1 (January 2023)