Inhibitors | Comment | Organism | Structure |
---|---|---|---|
9-deazaadenine-9-methylene-N-hydroxypyrrolidine | transition state analogue mimics of small oligonucleotide substrates of saporin-L1, e.g. A10 or A14 RNA or cyclic oxime GAGA, are powerful, slow-onset and tight-binding inhibitors when adenosine is replaced with the transition state mimic 9-deazaadenine-9-methylene-N-hydroxypyrrolidine. The inhibitors bind up to 40000fold tighter than RNA substrates and prevent saporin-L1 inhibition of rabbit reticulocyte translation. Saporin-S6 is resistant to inhibition by the compounds, overview | Saponaria officinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.082 | - |
cyclic oxime GAGA | pH 7.7, 20°C | Saponaria officinalis | |
0.095 | - |
A-10 RNA | pH 7.7, 20°C | Saponaria officinalis | |
0.266 | - |
linear GAGA | pH 7.7, 20°C | Saponaria officinalis | |
0.36 | - |
A-10 RNA | saporin-S6, pH 7.7, 20°C | Saponaria officinalis | |
0.639 | - |
Poly(A) | pH 7.8, 20°C | Saponaria officinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saponaria officinalis | depurination of the ribosomal sarcin-ricin tetraloop, GAGA, causing inhibition of protein synthesis and cellular death. Saporin-L1 shows robust activity against defined nucleic acid substrates and mammalian ribosomes, saporin-S6 shows low activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saponaria officinalis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Saponaria officinalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
A-10 RNA + H2O | i.e. 5'-CGCGAGAGCG-3' | Saponaria officinalis | ? | - |
? | |
cyclic oxime GAGA + H2O | - |
Saponaria officinalis | ? | - |
? | |
linear GAGA + H2O | - |
Saponaria officinalis | ? | - |
? | |
additional information | depurination of the ribosomal sarcin-ricin tetraloop, GAGA, causing inhibition of protein synthesis and cellular death. Saporin-L1 shows robust activity against defined nucleic acid substrates and mammalian ribosomes, saporin-S6 shows low activity | Saponaria officinalis | ? | - |
? | |
additional information | depurination of the ribosomal sarcin-ricin tetraloop, GAGA | Saponaria officinalis | ? | - |
? | |
poly(A) + H2O | - |
Saponaria officinalis | ? | - |
? | |
rabbit ribosome 80S rRNA + H2O | good substrate for saporin-L1, low activity by saporin-S6 | Saponaria officinalis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribosome inactivating protein | - |
Saponaria officinalis |
RIP | - |
Saponaria officinalis |
saporin-L1 | - |
Saponaria officinalis |
saporin-S6 | - |
Saponaria officinalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Saponaria officinalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
A-10 RNA | saporin-S6, pH 7.7, 20°C | Saponaria officinalis | |
1.02 | - |
Poly(A) | saporin-L1, pH 7.8, 20°C | Saponaria officinalis | |
4.88 | - |
linear GAGA | saporin-L1, pH 7.7, 20°C | Saponaria officinalis | |
5.01 | - |
cyclic oxime GAGA | saporin-L1, pH 7.7, 20°C | Saponaria officinalis | |
7.33 | - |
A-10 RNA | saporin-L1, pH 7.7, 20°C | Saponaria officinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Saponaria officinalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.0000023-0.0000087, GAGA stem-loop oligonucleotide inhibitors containing DADMeA, saporin-L1, pH 7.7, 20°C | Saponaria officinalis | |
additional information | - |
additional information | inhibition kinetics, sopain-L1, overview | Saponaria officinalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
A-10 RNA | saporin-S6, pH 7.7, 20°C | Saponaria officinalis | |
1.6 | - |
Poly(A) | pH 7.8, 20°C | Saponaria officinalis | |
18 | - |
linear GAGA | pH 7.7, 20°C | Saponaria officinalis | |
61 | - |
cyclic oxime GAGA | pH 7.7, 20°C | Saponaria officinalis | |
77 | - |
A-10 RNA | pH 7.7, 20°C | Saponaria officinalis |