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Literature summary for 3.2.2.22 extracted from

  • Sturm, M.B.; Tyler, P.C.; Evans, G.B.; Schramm, V.L.
    Transition state analogues rescue ribosomes from saporin-L1 ribosome inactivating protein (2009), Biochemistry, 48, 9941-9948.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
9-deazaadenine-9-methylene-N-hydroxypyrrolidine transition state analogue mimics of small oligonucleotide substrates of saporin-L1, e.g. A10 or A14 RNA or cyclic oxime GAGA, are powerful, slow-onset and tight-binding inhibitors when adenosine is replaced with the transition state mimic 9-deazaadenine-9-methylene-N-hydroxypyrrolidine. The inhibitors bind up to 40000fold tighter than RNA substrates and prevent saporin-L1 inhibition of rabbit reticulocyte translation. Saporin-S6 is resistant to inhibition by the compounds, overview Saponaria officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.082
-
cyclic oxime GAGA pH 7.7, 20°C Saponaria officinalis
0.095
-
A-10 RNA pH 7.7, 20°C Saponaria officinalis
0.266
-
linear GAGA pH 7.7, 20°C Saponaria officinalis
0.36
-
A-10 RNA saporin-S6, pH 7.7, 20°C Saponaria officinalis
0.639
-
Poly(A) pH 7.8, 20°C Saponaria officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saponaria officinalis depurination of the ribosomal sarcin-ricin tetraloop, GAGA, causing inhibition of protein synthesis and cellular death. Saporin-L1 shows robust activity against defined nucleic acid substrates and mammalian ribosomes, saporin-S6 shows low activity ?
-
?

Organism

Organism UniProt Comment Textmining
Saponaria officinalis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Saponaria officinalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
A-10 RNA + H2O i.e. 5'-CGCGAGAGCG-3' Saponaria officinalis ?
-
?
cyclic oxime GAGA + H2O
-
Saponaria officinalis ?
-
?
linear GAGA + H2O
-
Saponaria officinalis ?
-
?
additional information depurination of the ribosomal sarcin-ricin tetraloop, GAGA, causing inhibition of protein synthesis and cellular death. Saporin-L1 shows robust activity against defined nucleic acid substrates and mammalian ribosomes, saporin-S6 shows low activity Saponaria officinalis ?
-
?
additional information depurination of the ribosomal sarcin-ricin tetraloop, GAGA Saponaria officinalis ?
-
?
poly(A) + H2O
-
Saponaria officinalis ?
-
?
rabbit ribosome 80S rRNA + H2O good substrate for saporin-L1, low activity by saporin-S6 Saponaria officinalis ?
-
?

Synonyms

Synonyms Comment Organism
ribosome inactivating protein
-
Saponaria officinalis
RIP
-
Saponaria officinalis
saporin-L1
-
Saponaria officinalis
saporin-S6
-
Saponaria officinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Saponaria officinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.058
-
A-10 RNA saporin-S6, pH 7.7, 20°C Saponaria officinalis
1.02
-
Poly(A) saporin-L1, pH 7.8, 20°C Saponaria officinalis
4.88
-
linear GAGA saporin-L1, pH 7.7, 20°C Saponaria officinalis
5.01
-
cyclic oxime GAGA saporin-L1, pH 7.7, 20°C Saponaria officinalis
7.33
-
A-10 RNA saporin-L1, pH 7.7, 20°C Saponaria officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
assay at Saponaria officinalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information 0.0000023-0.0000087, GAGA stem-loop oligonucleotide inhibitors containing DADMeA, saporin-L1, pH 7.7, 20°C Saponaria officinalis
additional information
-
additional information inhibition kinetics, sopain-L1, overview Saponaria officinalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.016
-
A-10 RNA saporin-S6, pH 7.7, 20°C Saponaria officinalis
1.6
-
Poly(A) pH 7.8, 20°C Saponaria officinalis
18
-
linear GAGA pH 7.7, 20°C Saponaria officinalis
61
-
cyclic oxime GAGA pH 7.7, 20°C Saponaria officinalis
77
-
A-10 RNA pH 7.7, 20°C Saponaria officinalis