Literature summary for 3.2.2.22 extracted from

Tosi, G.; Fermani, S.; Falini, G.; Polito, L.; Bortolotti, M.; Bolognesi, A.
Crystallization and preliminary X-ray diffraction data analysis of stenodactylin, a highly toxic type 2 ribosome-inactivating protein from Adenia stenodactyla (2010), Acta Crystallogr. Sect. F, 66, 51-53.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified native stenodactylin, sitting drop vapour diffusion technique, mixing of 0.004 ml of 1.4-1.5 M sodium malonate, pH 6.8-7.5, reservoir solution with 0.004 ml of protein solution containing with 5.76 mg/ml protein in 5 mM sodium phosphate buffer, pH 7.0, 0.14 M NaCl and 4 mM galactose, equilibration against 0.75 ml of reservoir solution, 20°C, 3 weeks, X-ray diffraction structure determination and analysis at 2.15 A resolution	Adenia stenodactyla

Crystallization (Comment)

Organism

purified native stenodactylin, sitting drop vapour diffusion technique, mixing of 0.004 ml of 1.4-1.5 M sodium malonate, pH 6.8-7.5, reservoir solution with 0.004 ml of protein solution containing with 5.76 mg/ml protein in 5 mM sodium phosphate buffer, pH 7.0, 0.14 M NaCl and 4 mM galactose, equilibration against 0.75 ml of reservoir solution, 20°C, 3 weeks, X-ray diffraction structure determination and analysis at 2.15 A resolution

Adenia stenodactyla

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Adenia stenodactyla	ribosome-inactivating proteins, RIPs, are specific RNA N-glycosidases and inhibit protein synthesis and induce cell death by removing a single adenine from a specific rRNA loop. Type 1 RIPs are single-chain enzymes with N-glycosidase activity. Type 2 RIPs contain two chains A and B linked by a disulfide bond. The A chain has RIP enzymatic activity, whereas the B chain shows lectin activity and is able to bind to glycosylated receptors on the cell surface. Stenodactylin is a type 2 RIP	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Adenia stenodactyla	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme by ammonium sulfate fractionation and gel filtration	Adenia stenodactyla

Source Tissue

Source Tissue	Comment	Organism	Textmining
stem	-	Adenia stenodactyla	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	ribosome-inactivating proteins, RIPs, are specific RNA N-glycosidases and inhibit protein synthesis and induce cell death by removing a single adenine from a specific rRNA loop. Type 1 RIPs are single-chain enzymes with N-glycosidase activity. Type 2 RIPs contain two chains A and B linked by a disulfide bond. The A chain has RIP enzymatic activity, whereas the B chain shows lectin activity and is able to bind to glycosylated receptors on the cell surface. Stenodactylin is a type 2 RIP	Adenia stenodactyla	?	-	?

Synonyms

Synonyms	Comment	Organism
ribosome-inactivating protein	-	Adenia stenodactyla
RIP	-	Adenia stenodactyla
RNA N-glycosidase	-	Adenia stenodactyla
stenodactylin	-	Adenia stenodactyla
type 2RIP	-	Adenia stenodactyla

General Information

General Information	Comment	Organism
additional information	the toxicity of RIPs to cells and consequently to animals is highly variable and depends on their structure: in general, type 1 RIPs have a lower toxicity than type 2 RIPs	Adenia stenodactyla