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Literature summary for 3.2.2.22 extracted from
- Detection of intact ricin in crude and purified extracts from castor beans using matrix-assisted laser desorption ionization mass spectrometry (2009), Anal. Chem., 81, 1529-1535.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | ricin identification in food (milk, apple juice) or clinical samples (serum, saliva) with DNA substrate that mimics the natural RNA target combined with MALDI-TOF analysis of DNA to identify depurination (1-5 pmol ricin detectable), and tryptic digestion of ricin and analysis with LC-MS/MS | Ricinus communis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immunoaffinity capture with antiricinus communis agglutinin, immobilized and cross-linked to Dynabeads Protein G | Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GCGCGAGAGCGC + H2O | DNA substrate (100 pmol/microl) mimicking natural rRNA substrate, reaction buffer: 10 mM ammonium citrate with 1 mM ethylenediaminetetraacetic acid, pH 4, 37°C, 4 h | Ricinus communis | GCGCGGAGCGC + ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ricin | - |
Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Ricinus communis |
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Release 2023.1 (January 2023)
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