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Literature summary for 3.2.2.22 extracted from

  • Marsden, C.J.; Knight, S.; Smith, D.C.; Day, P.J.; Roberts, L.M.; Phillips, G.J.; Lord, J.M.
    Insertional mutagenesis of ricin A chain: a novel route to an anti-ricin vaccine (2004), Vaccine, 22, 2800-2805.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant ricin A chains in Escherichia coli strain JM101 Ricinus communis

Protein Variants

Protein Variants Comment Organism
additional information construction of mutant ricin A chains by insertional mutagenesis, the mutants possessing the specific 25-residue inserted internal peptide, derived from maize proRIP, show about 300fold reduced catalytic activity Ricinus communis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ricinus communis the wild-type holotoxin ricin is lethal for the target cell, e.g. Verocells, due to inactivation of ribosomes and inhibition of protein synthesis ?
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?

Organism

Organism UniProt Comment Textmining
Ricinus communis
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Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant ricin A chains from Escherichia coli strain JM101 by anion exchange chromatography and cobalt affinity chromatography to homogeneity Ricinus communis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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protein synthesis inhibition and cytotoxicity of wild-type and mutant ricin A chains, overview Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the wild-type holotoxin ricin is lethal for the target cell, e.g. Verocells, due to inactivation of ribosomes and inhibition of protein synthesis Ricinus communis ?
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?
ribosomes + H2O substrate from yeast Ricinus communis ?
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rRNA + H2O product determination using aniline coupling Ricinus communis ?
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?

Subunits

Subunits Comment Organism
More the heterodimeric enzyme consists of the catalytic ricin A chain, RTA, and the cabohydrate-binding B chain, RTB Ricinus communis

Synonyms

Synonyms Comment Organism
ricin
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Ricinus communis
RIPII
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Ricinus communis
type II ribosome-inactivating protein
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Ricinus communis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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in vitro assay at, substrate yeast ribosomes Ricinus communis
37
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in vivo assay at, in Vero cells Ricinus communis