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Literature summary for 3.2.2.22 extracted from
- Isolation and characterization of an RIP (ribosome-inactivating protein)-like protein from tobacco with dual enzymatic activity (2004), Plant Physiol., 134, 171-181.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 26000, SDS-PAGE | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Nicotiana tabacum | the enzyme inhibits translation of rabbit reticulocytes and wheat germ lysate, the enzyme shows anti-microbial activity, e.g. against Trichoderma reesei, Ervinia amylovora, Shigella asonei, Salmonella typhimurium, Rhizobium leguminosarum, Cytospora cankar, and Pseudomonas solancearum, overview | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from leaves by ion exchange chromatography and gel filtration | Nicotiana tabacum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Nicotiana tabacum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
adenine releasing activity with substrates hering sperm DNA, rRNA, and tRNA, depurination activity with ribosome substrate from Artemia sp. and rat | Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Artemia sp. ribosome + H2O | depurination of rRNA | Nicotiana tabacum | ? | - |
? | |
| hering sperm DNA + H2O | adenine releasing activity | Nicotiana tabacum | ? | - |
? | |
| additional information | the enzyme inhibits translation of rabbit reticulocytes and wheat germ lysate, the enzyme shows anti-microbial activity, e.g. against Trichoderma reesei, Ervinia amylovora, Shigella asonei, Salmonella typhimurium, Rhizobium leguminosarum, Cytospora cankar, and Pseudomonas solancearum, overview | Nicotiana tabacum | ? | - |
? | |
| rat ribosome + H2O | depurination of rRNA | Nicotiana tabacum | ? | - |
? | |
| tRNA + H2O | adenine releasing activity | Nicotiana tabacum | ? | - |
? | |
| yeast ribosome + H2O | depurination of rRNA | Nicotiana tabacum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26000, SDS-PAGE | Nicotiana tabacum |
| More | the N-terminal amino acid sequence of the enzyme is identical with that of several superoxide dimutases | Nicotiana tabacum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (ribosome-inactivating protein)-like protein | - |
Nicotiana tabacum |
| TRIP | - |
Nicotiana tabacum |
| type I RIP | - |
Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Nicotiana tabacum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | the pH preference depends on the substrate type, overview | Nicotiana tabacum |
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Release 2023.1 (January 2023)
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