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Literature summary for 3.2.2.22 extracted from

  • Bass, H.W.; Krawetz, J.E.; GR, O.B.; Zinselmeier, C.; Habben, J.E.; Boston, R.S.
    Maize ribosome-inactivating proteins (RIPs) with distinct expression patterns have similar requirements for proenzyme activation (2004), J. Exp. Bot., 55, 2219-2233.
    View publication on PubMed
Search for more literature for 3.2.2.22

Activating Compound

Activating Compound Comment Organism Structure
additional information expression of gene RIP3:2 is up-regulated by drought Zea mays

Cloned(Commentary)

Cloned (Comment) Organism
gene RIP3:1 is expressed under control of the Opaque-2 promoter Zea mays
gene RIP3:2, genomic DNA library screening, DNA and amino acid sequence determination and analyis, RNA mapping and expression profiling, expression is not under control of the Opaque-2 promoter, expression of gene RIP3:2 encoding isozyme RIP2 in Escherichia coli strain BL21(DE3) as N-terminally His-tagged protein Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the isozyme accumulates as inactive precursor protein Zea mays
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Zea mays the isozyme RIP1 has defense-related function in maize plants ?
-
 ?
additional information Zea mays the isozyme RIP2 has defense-related function in maize plants ?
-
 ?
rRNA + H2O Zea mays inhibition of translation and protein synthesis, cytotoxic ?
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays
-
 strains W64A and W64A o2, gene RIP3:2 encoding the type 3 RIP isozyme RIP2
-
Zea mays P25891 strains W64A and W64A o2, gene RIP3:1 encoding the type 3 RIP isozyme RIP1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification isozyme RIP1 is synthesized as inactive precursor protein B-32 or proRIP, which is activated by proteolytic cleavage into two polypeptide chains forming the dimeric enzyme, the enzyme can be activated by papain Zea mays
proteolytic modification isozyme RIP2 is synthesized as inactive precursor protein proRIP2, which is activated by proteolytic cleavage into two polypeptide chains forming the dimeric enzyme, the enzyme can be activated by papain Zea mays

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged isozyme RIP2 from Escherichia coli strain BL21(DE3) Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Zea mays
-
kernel
-
 Zea mays
-
leaf
-
 Zea mays
-
root
-
 Zea mays
-
shoot
-
 Zea mays
-
silk
-
 Zea mays
-
tassel
-
 Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the isozyme RIP1 has defense-related function in maize plants Zea mays ?
-
 ?
additional information the isozyme RIP2 has defense-related function in maize plants Zea mays ?
-
 ?
additional information isozyme RIP1 shows relatively low cytotoxic activity and ressembles type I RIPs concerning its substrate specificity Zea mays ?
-
 ?
additional information isozyme RIP2 shows relatively low cytotoxic activity and ressembles type I RIPs concerning its substrate specificity Zea mays ?
-
 ?
rRNA + H2O inhibition of translation and protein synthesis, cytotoxic Zea mays ?
-
 ?
rRNA + H2O depurination of specific sites in rRNA Zea mays ?
-
 ?

Subunits

Subunits Comment Organism
dimer type III RIP isozyme RIP1 forms dimers and ressembles type II RIPs concerning its subunit structure Zea mays
dimer type III RIP isozyme RIP2 forms dimers and ressembles type II RIPs concerning its subunit structure Zea mays

Synonyms

Synonyms Comment Organism
B-32
-
 Zea mays
ribosome-inactivating protein 3
-
 Zea mays
RIP1
-
 Zea mays
RIP2
-
 Zea mays
RNA-specific N-glycosidase
-
 Zea mays
rRNA N-glycosidase
-
 Zea mays
type III ribosome-inactivating protein
-
 Zea mays
type III RIP
-
 Zea mays