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Literature summary for 3.2.2.22 extracted from
- Substrate specificity of a maize ribosome-inactivating protein differs across diverse taxa (2000), Eur. J. Biochem., 267, 1966-1974.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an active deletion mutant MOD1 of recombinant proRIP1 with reduced activity compared to recombinant RIP1 after activation of both by papain | Zea mays |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Zea mays |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| large rRNA + H2O | Zea mays | inhibition of translation and protein synthesis, cytotoxic | ? | - |
? | |
| additional information | Zea mays | the isozyme RIP1 has defense-related function in maize plants | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | P25891 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | isozyme RIP1 is synthesized as inactive precursor protein B-32 or proRIP, which is activated by proteolytic cleavage into two polypeptide chains forming the dimeric enzyme, the enzyme can be activated by papain | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Zea mays | - |
| kernel | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| large rRNA + H2O | inhibition of translation and protein synthesis, cytotoxic | Zea mays | ? | - |
? | |
| large rRNA + H2O | substrate are intact rabbit ribosomes, best substrate, or ribosomes from Aspergillus flavus, low activity, or Zea mays, the latter are poor substrates, depurination of specific sites in rRNA, formation of RNA fragments | Zea mays | large rRNA fragments, partially deadenylated | fragment size overview, product determination using aniline coupling | ? | |
| additional information | the isozyme RIP1 has defense-related function in maize plants | Zea mays | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| B-32 | - |
Zea mays |
| ribosome-inactivating protein 3 | - |
Zea mays |
| RIP1 | - |
Zea mays |
| RNA-specific N-glycosidase | - |
Zea mays |
| rRNA N-glycosidase | - |
Zea mays |
| type 3 RIP | - |
Zea mays |
| type III ribosome-inactivating protein | - |
Zea mays |
| type III RIP | - |
Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Zea mays |
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Release 2023.1 (January 2023)
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