Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.22 extracted from

  • Parikh, B.A.; Baykal, U.; Di, R.; Tumer, N.E.
    Evidence for retro-translocation of pokeweed antiviral protein from endoplasmic reticulum into cytosol and separation of its activity on ribosomes from its activity on capped RNA (2005), Biochemistry, 44, 2478-2490.
    View publication on PubMed
Search for more literature for 3.2.2.22

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant PAPs in Escherichia coli strain BL21(DE3) Phytolacca americana

Protein Variants

Protein Variants Comment Organism
E176V site-directed mutagenesis, inactive mutant showing no cytotoxicity Phytolacca americana
N70A site-directed mutagenesis, the mutation delays the enzyme depurination activity on ribosomes and the onset of translation arrest, N70A PAP shows highly reduced cytotoxicity and destabilizes its own mRNA, but binds to cap and blocks cap-dependent translation Phytolacca americana

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme is synthesized as inactive precursor protein, accumulated in the endoplasmic reticulum associated to membranes, and needs to cleaved to the mature active form in the endoplasmic reticulum of the targeted cell, the mature form is retro-translocated from the ER to the cytosol where it is accumulated and escapes degradation, residues E176 and N70 are essential for catalytic activity Phytolacca americana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Phytolacca americana PAP causes translation arrest and is cytotoxic in targeted cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Phytolacca americana P10297 pokeweed
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme is synthesized as inactive precursor protein and needs to cleaved to the mature active form in the endoplasmic reticulum of the targeted cell, the mature form is retro-translocated from the ER to the cytosol where it escapes degradation Phytolacca americana

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant PAPs from Escherichia coli strain BL21(DE3) by m7G affinity chromatography Phytolacca americana

Reaction

Reaction Comment Organism Reaction ID
A4324 in 28S rRNA + H2O = deadenylated 28S rRNA + adenine N70 of PAP is required for depurination catalytic activity, N70 facilitates depurination in trans Phytolacca americana
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Phytolacca americana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rRNA + H2O PAP causes translation arrest and is cytotoxic in targeted cells Phytolacca americana ?
-
 ?
rRNA + H2O pokeweed antiviral protein binds to ribosomes and depurinates a specific adenine in the highly conserved alpha-sarcin/ricin loop of the large subunit rRNA Phytolacca americana ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Phytolacca americana

Synonyms

Synonyms Comment Organism
PAP
-
 Phytolacca americana
pokeweed antiviral protein
-
 Phytolacca americana
type I ribosome-inactivating protein
-
 Phytolacca americana
type I RIP
-
 Phytolacca americana