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Literature summary for 3.2.2.22 extracted from

  • Brigotti, M.; Carnicelli, D.; Alvergna, P.; Mazzaracchio, R.; Sperti, S.; Montanaro, L.
    The RNA-N-glycosidase activity of Shiga-like toxin I: kinetic parameters of the native and activated toxin (1997), Toxicon, 35, 1431-1437.
    View publication on PubMed
Search for more literature for 3.2.2.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00084
-
 rRNA pH 7.4, 28°C, Artemia salina ribosomes, untreated holotoxin Escherichia coli
0.00091
-
 rRNA pH 7.4, 28°C, Artemia salina ribosomes, untreated A subunit Escherichia coli
0.00096
-
 rRNA pH 7.4, 28°C, Artemia salina ribosomes, A subunit treated with urea and dithiothreitol Escherichia coli
0.00098
-
 rRNA pH 7.4, 28°C, Artemia salina ribosomes, holotoxin treated with trypsin, urea and dithiothreitol Escherichia coli
0.00137
-
 rRNA pH 7.4, 28°C, Artemia salina ribosomes, A subunit treated with trypsin, urea and dithiothreitol Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 C600(H19J), shiga-like toxin I
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information releases adenine from highly purified Artemia salina ribosomes Escherichia coli ?
-
 ?
rRNA + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
shiga-like toxin I
-
 Escherichia coli
SLT-I
-
 Escherichia coli
SLT-II
-
 Escherichia coli
SLT-IIv
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 rRNA pH 7.4, 28°C, Artemia salina ribosomes, untreated holotoxin Escherichia coli
0.0933
-
 rRNA pH 7.4, 28°C, Artemia salina ribosomes, untreated A subunit Escherichia coli
10.7
-
 rRNA pH 7.4, 28°C, Artemia salina ribosomes, A subunit treated with trypsin, urea and dithiothreitol Escherichia coli
11.6
-
 rRNA pH 7.4, 28°C, Artemia salina ribosomes, A subunit treated with urea and dithiothreitol Escherichia coli
12.8
-
 rRNA pH 7.4, 28°C, Artemia salina ribosomes, holotoxin treated with trypsin, urea and dithiothreitol Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information SLT-I ans its A subunit are cofactor-independent Escherichia coli