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Literature summary for 3.2.2.22 extracted from
- Purification, characterization and subcellular localization of a type-1 ribosome-inactivating protein from the sarcocarp of Cucurbita pepo (1996), Eur. J. Biochem., 242, 585-591.
General Stability
| General Stability | Organism |
|---|---|
| retains activity upon freeze-thawing | Cucurbita pepo |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
gel filtration | Cucurbita pepo |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rRNA + H2O | Cucurbita pepo | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucurbita pepo | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cucurbita pepo |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Cucurbita pepo | - |
| leaf | intercellular space | Cucurbita pepo | - |
| sarcocarp | - |
Cucurbita pepo | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity 2500000 U/mg, 1 unit is the amount of enzyme causing 50% inhibition in 1 ml | Cucurbita pepo |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified protein is stable for at least 6 months | Cucurbita pepo |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | also active on wheat-germ ribosomes and Escherichia coli ribosomes | Cucurbita pepo | ? | - |
? | |
| rRNA + H2O | - |
Cucurbita pepo | ? | - |
? | |
| rRNA + H2O | pepocin cleaves the N-glycosidic bond at A4324 in rabbit 28S rRNA | Cucurbita pepo | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pepocin | - |
Cucurbita pepo |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cucurbita pepo | isoelectric focusing | - |
9.9 |
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