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Literature summary for 3.2.2.22 extracted from

  • Bolognesi, A.; Olivieri, F.; Battelli, M.G.; Barbieri, L.; Falasca, A.I.; Parente, A.; Del Vecchio Blanco, F.; Stirpe, F.
    Ribosome-inactivating proteins (RNA N-glycosidases) from the seeds of Saponaria ocymoides and Vaccaria pyramidata (1995), Eur. J. Biochem., 228, 935-940.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27700
-
1 * 27700, SDS-PAGE Gypsophila vaccaria
28000
-
gel filtration Gypsophila vaccaria
30200
-
gel filtration Saponaria ocymoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Saponaria ocymoides
-
?
-
?
rRNA + H2O Gypsophila vaccaria
-
?
-
?

Organism

Organism UniProt Comment Textmining
Gypsophila vaccaria
-
-
-
Saponaria ocymoides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saponaria ocymoides
-
Gypsophila vaccaria

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Saponaria ocymoides
-
seed
-
Gypsophila vaccaria
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity 740700 U/mg, 1 U is the amount of enzyme causing 50% inhibition in 1 ml of rabbit-reticulocyte lysate Saponaria ocymoides
additional information
-
specific activity 401600 U/mg, I U is the amount of enzyme causing 50% inhibition in 1 ml of rabbit-reticulocyte lysate Gypsophila vaccaria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information inhibits protein synthesis by a rabbit-reticulocyte lysate, smaller effect on poly(U)-directed phenylalanine polymerisation by rat liver ribosomes and on protein synthesis by various cell lines Saponaria ocymoides ?
-
?
additional information inhibits protein synthesis by a rabbit-reticulocyte lysate, smaller effect on poly(U)-directed phenylalanine polymerisation by rat liver ribosomes and on protein synthesis by various cell lines Gypsophila vaccaria ?
-
?
additional information damages eukaryotic ribosomes by cleaving the N-glycosidic bond at A4324 of the 28 S rRNA Saponaria ocymoides ?
-
?
additional information damages eukaryotic ribosomes by cleaving the N-glycosidic bond at A4324 of the 28 S rRNA Gypsophila vaccaria ?
-
?
rRNA + H2O
-
Saponaria ocymoides ?
-
?
rRNA + H2O
-
Gypsophila vaccaria ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 27700, SDS-PAGE Gypsophila vaccaria
monomer 1 * 30200, SDS-PAGE Saponaria ocymoides

pI Value

Organism Comment pI Value Maximum pI Value
Saponaria ocymoides pI greater than 9.5
-
additional information
Gypsophila vaccaria pI greater than 9.5
-
additional information