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Literature summary for 3.2.2.21 extracted from

  • Moe, E.; Hall, D.R.; Leiros, I.; Monsen, V.T.; Timmins, J.; McSweeney, S.
    Structure-function studies of an unusual 3-methyladenine DNA glycosylase II (AlkA) from Deinococcus radiodurans (2012), Acta Crystallogr. Sect. D, 68, 703-712.
    View publication on PubMed
Search for more literature for 3.2.2.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Codon Plus cells Deinococcus radiodurans

Crystallization (Commentary)

Crystallization (Comment) Organism
isoform AlkA2, hanging drop vapor diffusion method, using 1 M LiCl2, 0.1 M MES pH 6.0, 10% (w/v) PEG 6000 Deinococcus radiodurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Deinococcus radiodurans
-
 3-methyladenine + 7-methylguanine + DNA
-
 ?
additional information Deinococcus radiodurans the enzyme isoform AlkA2 possesses no activity towards 1,N6-ethenoadenine and hypoxanthine in alkylated DNA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans Q9RRB0
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-loaded HisTrap column chromatography Deinococcus radiodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alkylated DNA + H2O
-
 Deinococcus radiodurans 3-methyladenine + 7-methylguanine + DNA
-
 ?
additional information the enzyme isoform AlkA2 possesses no activity towards 1,N6-ethenoadenine and hypoxanthine in alkylated DNA Deinococcus radiodurans ?
-
 ?

Synonyms

Synonyms Comment Organism
3-methyladenine DANN glycosylase II
-
 Deinococcus radiodurans
AlkA1 isoform Deinococcus radiodurans
AlkA2 isoform Deinococcus radiodurans