Literature summary for 3.2.2.21 extracted from

Rubinson, E.H.; Metz, A.H.; OQuin, J.; Eichman, B.F.
A new protein architecture for processing alkylation damaged DNA: the crystal structure of DNA glycosylase AlkD (2008), J. Mol. Biol., 381, 13-23.

Search for more literature for 3.2.2.21

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli HMS174 cells	Bacillus cereus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method, with 85 mM HEPES (pH 7.5), 15% (w/v) PEG 4000, and 17% (v/v) glycerol, at 21°C	Bacillus cereus

Protein Variants

Protein Variants	Comment	Organism
D113N	the mutation results in a 100fold decrease in the single-turnover rate of 7-methylguanine excision relative to the wild type enzyme	Bacillus cereus
R148A	the mutation results in a 100fold decrease in the single-turnover rate of 7-methylguanine excision relative to the wild type enzyme	Bacillus cereus

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	-	strain ATCC 14579	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, heparin affinity column chromatography, and gel filtration	Bacillus cereus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alkylated DNA + H2O	-	Bacillus cereus	3-methyladenine + 7-methylguanine + DNA	-	?
additional information	AlkD does not excise 1,N6-ethenoadenine	Bacillus cereus	?	-	?

Synonyms

Synonyms	Comment	Organism
AlkD	-	Bacillus cereus
DNA glycosylase	-	Bacillus cereus

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Release 2023.1 (January 2023)