Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli HMS174 cells | Bacillus cereus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method, with 85 mM HEPES (pH 7.5), 15% (w/v) PEG 4000, and 17% (v/v) glycerol, at 21°C | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
D113N | the mutation results in a 100fold decrease in the single-turnover rate of 7-methylguanine excision relative to the wild type enzyme | Bacillus cereus |
R148A | the mutation results in a 100fold decrease in the single-turnover rate of 7-methylguanine excision relative to the wild type enzyme | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
strain ATCC 14579 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, heparin affinity column chromatography, and gel filtration | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylated DNA + H2O | - |
Bacillus cereus | 3-methyladenine + 7-methylguanine + DNA | - |
? | |
additional information | AlkD does not excise 1,N6-ethenoadenine | Bacillus cereus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlkD | - |
Bacillus cereus |
DNA glycosylase | - |
Bacillus cereus |