KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000014 | - |
alkylated DNA | pH 7.5, 37°C, excision of 5-hydroxymethyluracil, denatured DNA | Escherichia coli | |
0.0000044 | - |
alkylated DNA | pH 7.5, 37°C, excision of 5-hydroxymethyluracil | Escherichia coli | |
0.0000108 | - |
alkylated DNA | pH 7.5, 37°C, excision of 3-methyladenine | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylated DNA + H2O | Escherichia coli | DNA repair enzyme, inducible base excision repair pathway of DNA alkylation damage | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0247 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, purified enzyme remains stable for more than 4 years when stored in 20% glycerol, | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
alkylated DNA + H2O | DNA repair enzyme, inducible base excision repair pathway of DNA alkylation damage | Escherichia coli | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
additional information | enzyme also excises thymine residues oxidized in the methyl group like 5-formyluracil and 5-hydroxymethyluracil | Escherichia coli | ? | - |
? |