Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermidine | stimulates activity | Escherichia coli | |
spermine | stimulates activity | Escherichia coli |
General Stability | Organism |
---|---|
very unstable | Micrococcus luteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | - |
Micrococcus luteus | |
additional information | resistant to product inhibition by free 3-methyladenine | Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
N-ethylmaleimide | - |
Micrococcus luteus | |
NaCl | - |
Escherichia coli | |
p-mercuribenzoate | - |
Micrococcus luteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | no obligatory requirement, but addition of 1.5 mM MgCl2 causes stimulation in the catalytic activity 2fold | Micrococcus luteus | |
Mg2+ | stimulated by 1.5 mM MgCl2 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
gel filtration, Svedberg equation | Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylated DNA + H2O | Escherichia coli | - |
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
alkylated DNA + H2O | Micrococcus luteus | cellular repair of alkylated DNA base modifications | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Micrococcus luteus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Micrococcus luteus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000375 | - |
- |
Micrococcus luteus |
Storage Stability | Organism |
---|---|
-20°C, stored in 50% glycerol, undergoes complete inactivation in 3-4 weeks | Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
alkylated DNA + H2O | - |
Micrococcus luteus | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
alkylated DNA + H2O | cellular repair of alkylated DNA base modifications | Micrococcus luteus | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
alkylated DNA + H2O | - |
Micrococcus luteus | 3-methyladenine + 3-methylguanine + 7-methyladenine + 7-methylguanine + DNA | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | no activity on O2-methylcytosine, O2-methylthymine, O4-methylthymine or O6-methylguanine | Micrococcus luteus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
65 min time for 50% inactivation | Escherichia coli |
50 | - |
3.5 min, 50% inactivation | Micrococcus luteus |
60 | - |
heat stable, retains 40% of its original activity after 2 min incubation | Micrococcus luteus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no absolute cofactor requirement | Micrococcus luteus |