Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermidine | stimulates activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
apurinic-DNA | - |
Escherichia coli | |
Double-stranded DNA | - |
Escherichia coli | |
additional information | resistant to product inhibition by free 3-methyladenine | Escherichia coli | |
N-ethylmaleimide | 1 mM, 24% inhibition | Escherichia coli | |
NaCl | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000092 | - |
3-methyladenine residues in alkylated DNA | pH 8.0, 37°C, release of 3-methyladenine | Escherichia coli | |
0.000011 | - |
7-methylguanine residues in alkylated DNA | pH 8.0, 37°C, release of 7-methylguanine | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulated by 1 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylated DNA + H2O | Escherichia coli | - |
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli BW 9062 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyladenine residues in alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + DNA | - |
? | |
7-methylguanine residues in alkylated DNA + H2O | - |
Escherichia coli | 7-methylguanine + DNA | - |
? | |
alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + 7-methylguanine + 7-methyladenine + DNA | - |
? | |
DNA treated with beta-[3H]propiolactone + H2O | - |
Escherichia coli | N1-(carboxyethyl)adenine + N7-(carboxymethyl)guanine | - |
? | |
additional information | enzyme releases N1-(carboxyethyl)adenine and N7-(carboxymethyl)guanine from DNA treated with beta-propiolactone, but does not release the aflatoxin B-1 adduct at N-7 of guanine, release of 3-methyladenine from single-stranded DNA is 9% of the rate from double-stranded DNA, enzyme will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened | Escherichia coli | ? | - |
? | |
additional information | enzyme releases N1-(carboxyethyl)adenine and N7-(carboxymethyl)guanine from DNA treated with beta-propiolactone, but does not release the aflatoxin B-1 adduct at N-7 of guanine, release of 3-methyladenine from single-stranded DNA is 9% of the rate from double-stranded DNA, enzyme will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened | Escherichia coli BW 9062 | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
50% inactivation requires 65 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | 7.4 | 7.3 |