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Literature summary for 3.2.2.21 extracted from

  • Thomas, L.; Yang, C.H.; Goldthwait, D.A.
    Two DNA glycosylases in Escherichia coli which release primarily 3-methyladenine (1982), Biochemistry, 21, 1162-1169.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
spermidine stimulates activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
apurinic-DNA
-
Escherichia coli
Double-stranded DNA
-
Escherichia coli
additional information resistant to product inhibition by free 3-methyladenine Escherichia coli
N-ethylmaleimide 1 mM, 24% inhibition Escherichia coli
NaCl
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000092
-
3-methyladenine residues in alkylated DNA pH 8.0, 37°C, release of 3-methyladenine Escherichia coli
0.000011
-
7-methylguanine residues in alkylated DNA pH 8.0, 37°C, release of 7-methylguanine Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulated by 1 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Escherichia coli
-
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli BW 9062
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyladenine residues in alkylated DNA + H2O
-
Escherichia coli 3-methyladenine + DNA
-
?
7-methylguanine residues in alkylated DNA + H2O
-
Escherichia coli 7-methylguanine + DNA
-
?
alkylated DNA + H2O
-
Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
alkylated DNA + H2O
-
Escherichia coli 3-methyladenine + 7-methylguanine + 7-methyladenine + DNA
-
?
DNA treated with beta-[3H]propiolactone + H2O
-
Escherichia coli N1-(carboxyethyl)adenine + N7-(carboxymethyl)guanine
-
?
additional information enzyme releases N1-(carboxyethyl)adenine and N7-(carboxymethyl)guanine from DNA treated with beta-propiolactone, but does not release the aflatoxin B-1 adduct at N-7 of guanine, release of 3-methyladenine from single-stranded DNA is 9% of the rate from double-stranded DNA, enzyme will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened Escherichia coli ?
-
?
additional information enzyme releases N1-(carboxyethyl)adenine and N7-(carboxymethyl)guanine from DNA treated with beta-propiolactone, but does not release the aflatoxin B-1 adduct at N-7 of guanine, release of 3-methyladenine from single-stranded DNA is 9% of the rate from double-stranded DNA, enzyme will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened Escherichia coli BW 9062 ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
50% inactivation requires 65 min Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing 7.4 7.3