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Literature summary for 3.2.2.20 extracted from

  • Troll, C.J.; Adhikary, S.; Cueff, M.; Mitra, I.; Eichman, B.F.; Camps, M.
    Interplay between base excision repair activity and toxicity of 3-methyladenine DNA glycosylases in an E. coli complementation system (2014), Mutat. Res., 763-764, 64-73.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in in a glycosylase-deficient Escherichia coli strain MV1932 Saccharomyces cerevisiae
expressed in in a glycosylase-deficient Escherichia coli strain MV1932 Saccharomyces pombe

Protein Variants

Protein Variants Comment Organism
D170N the mutation substantially decreases enzymatic activity without abolishing it Saccharomyces pombe
I147V/T151A/A206S the mutant retains base excision activity toward alkylated DNA substrates, but its level of activity is decreased compared to the wild type Saccharomyces pombe
K126N/E130G/N175D the mutant retains base excision activity toward alkylated DNA substrates, but its level of activity is decreased compared to the wild type Saccharomyces pombe
L122M the mutant retains base excision activity toward alkylated DNA substrates, but its level of activity is decreased compared to the wild type Saccharomyces pombe
N42Y/V165A the mutant retains base excision activity toward alkylated DNA substrates, but its level of activity is decreased compared to the wild type Saccharomyces pombe
R149K the mutant retains base excision activity toward alkylated DNA substrates, but its level of activity is decreased compared to the wild type Saccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Saccharomyces cerevisiae highest activity by producing 3-methylguanine 3-methyladenine + ?
-
 ?
alkylated DNA + H2O Saccharomyces pombe highest activity by producing 3-methylguanine 3-methyladenine + ?
-
 ?
alkylated DNA + H2O Saccharomyces cerevisiae
-
 1,N6-ethenoadenine + ?
-
 ?
alkylated DNA + H2O Saccharomyces pombe
-
 1,N6-ethenoadenine + ?
-
 ?
alkylated DNA + H2O Saccharomyces cerevisiae
-
 7-methylguanine + ?
-
 ?
alkylated DNA + H2O Saccharomyces pombe
-
 7-methylguanine + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae
-
 Saccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alkylated DNA + H2O highest activity by producing 3-methylguanine Saccharomyces cerevisiae 3-methyladenine + ?
-
 ?
alkylated DNA + H2O highest activity by producing 3-methylguanine Saccharomyces pombe 3-methyladenine + ?
-
 ?
alkylated DNA + H2O
-
 Saccharomyces cerevisiae 1,N6-ethenoadenine + ?
-
 ?
alkylated DNA + H2O
-
 Saccharomyces pombe 1,N6-ethenoadenine + ?
-
 ?
alkylated DNA + H2O
-
 Saccharomyces cerevisiae 7-methylguanine + ?
-
 ?
alkylated DNA + H2O
-
 Saccharomyces pombe 7-methylguanine + ?
-
 ?

Synonyms

Synonyms Comment Organism
3MeA DNA glycosylase
-
 Saccharomyces cerevisiae
3MeA DNA glycosylase
-
 Saccharomyces pombe
MAG
-
 Saccharomyces cerevisiae
MAG1
-
 Saccharomyces pombe
N3-methyladenine DNA glycosylase
-
 Saccharomyces cerevisiae
N3-methyladenine DNA glycosylase
-
 Saccharomyces pombe

General Information

General Information Comment Organism
physiological function the enzyme activity is necessary for repair of endogenous alkyl lesions Saccharomyces cerevisiae
physiological function the enzyme activity is necessary for repair of endogenous alkyl lesions Saccharomyces pombe