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Literature summary for 3.2.2.20 extracted from
- Purification and characterization of 3-methyladenine DNA glycosylase I from Escherichia coli (1987), Nucleic Acids Res., 15, 2787-2801.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-Methylguanine | - |
Escherichia coli |  |
| adenine | - |
Escherichia coli | |
| Caffeine | - |
Escherichia coli | |
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing does not effect any apparent loss in enzyme activity | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Methyladenine | - |
Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| p-hydroxymercuribenzoate | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | CaCl2 stimulates enzyme activity | Escherichia coli | |
| Mg2+ | MgSO4 stimulates enzyme activity | Escherichia coli | |
| Mn2+ | MnCl2 stimulates enzyme activity | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21100 | - |
deduced from the nucleotide sequence of the tag gene | Escherichia coli |
| 22500 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alkylated DNA + H2O | Escherichia coli | - |
3-methyladenine + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 derivative MS23 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overproducing strain, carrying the tag gene on a multicopy plasmid | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0073 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stored frozen in buffer containing 1 mg/ml bovine serum albumin, stable for more than 6 months | Escherichia coli |
| -70°C, buffer containing 20% glycerol, stable for long term storage | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + ? | - |
? | |
| additional information | no detectable endonuclease activity on native, depurinated or alkylated plasmid DNA | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 22500, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TagI | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | no activity below pH 5.0 and above pH 10.0 | Escherichia coli |
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