Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.20 extracted from

  • Riazuddin, S.; Lindahl, T.
    Properties of 3-methyladenine-DNA glycosylase from Escherichia coli (1978), Biochemistry, 17, 2110-2118.
    View publication on PubMed
Search for more literature for 3.2.2.20

Inhibitors

Inhibitors Comment Organism Structure
3-Methyladenine product inhibition Escherichia coli
additional information free adenine, 1 methyladenine, 7-methylguanine, caffeine or uracil fails to inhibit the enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000006
-
 3-methyladenine residues in alkylated DNA pH 7.6, 37°C, release of 3-methyladenine Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slight stimulation about 30% with 5-15 mM MgCl2 Escherichia coli
additional information does not require divalent metal ions Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 sucrose densitiy gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Escherichia coli double-stranded DNA is an effective substrate, enzyme is less efficient in excision of base damage from single-stranded regions transiently formed in DNA during transcription and replication 3-methyladenine + ?
-
 ?
alkylated DNA + H2O Escherichia coli reaction of the enzyme with alkylated DNA leads to introduction of apurinic sites but no chain breaks 3-methyladenine + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0093
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyladenine residues in alkylated DNA + H2O
-
 Escherichia coli 3-methyladenine + ?
-
 ?
alkylated DNA + H2O
-
 Escherichia coli 3-methyladenine + ? releases 3-methyladenine and 3-ethyladenine ?
alkylated DNA + H2O double-stranded DNA is an effective substrate, enzyme is less efficient in excision of base damage from single-stranded regions transiently formed in DNA during transcription and replication Escherichia coli 3-methyladenine + ?
-
 ?
alkylated DNA + H2O reaction of the enzyme with alkylated DNA leads to introduction of apurinic sites but no chain breaks Escherichia coli 3-methyladenine + ?
-
 ?
additional information does not liberate 7-methylguanine, O6-methylguanine, 7-methyladenine, 7-ethylguanine, O6-ethylguanine, and arylalkylated purine derivatives obtained by treatment of DNA with 7-bromomethyl-12-methylbenz[alpha]anthracene, no detectable nuclease activity with native DNA, depurinated DNA, ultraviolet-irradiated DNA, or X-irradiated DNA as potential substrates, enzyme does not release hypoxanthine or xanthine in free form from by nitrous acid treatment partly demainated DNA Escherichia coli ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.8 similar activity in either HEPES-KOH or Tris-HCl buffers Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information no absolute cofactor requirement Escherichia coli