Literature summary for 3.2.1.86 extracted from

Yip, V.L.Y.; Varrot, A.; Davies, G.J.; Rajan, S.S.; Yang, X.; Thompson, J.; Anderson, W.F.; Withers, S.G.
An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima (2004), J. Am. Chem. Soc., 126, 8354-8355.

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Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	-	Thermotoga maritima
dithiothreitol	-	Thermotoga maritima
NAD+	0.001 mM for highest activity	Thermotoga maritima

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.041	-	p-nitrophenyl-beta-D-glucopyranoside 6-phosphate	pH 7.5	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	much less effective activator than Mn2+	Thermotoga maritima
Mg2+	much less effective activator than Mn2+	Thermotoga maritima
Mn2+	best activator	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cellobiose 6-phosphate + H2O	Thermotoga maritima	-	D-glucose 6-phosphate + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X108	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cellobiose 6-phosphate + H2O	-	Thermotoga maritima	D-glucose 6-phosphate + D-glucose	-	?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O	-	Thermotoga maritima	p-nitrophenol + D-glucopyranose 6-phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.9	-	p-nitrophenyl-beta-D-glucopyranoside 6-phosphate	pH 7.5	Thermotoga maritima

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Release 2023.1 (January 2023)