Application | Comment | Organism |
---|---|---|
food industry | the enzyme is considered as a candidate for application particularly in the animal feed industry | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 11% inhibition at 5 mM | Aspergillus niger | |
Co2+ | 52% inhibition at 5 mM | Aspergillus niger | |
Cu2+ | complete inhibition at 5 mM | Aspergillus niger | |
Fe2+ | complete inhibition at 5 mM | Aspergillus niger | |
Mn2+ | complete inhibition at 5 mM | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, 0.62 mg/ml with barley beta-glucan at pH 5.0, 60°C,and 0.38 mg/ml with lichenan at pH 5.0, 60°C | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus niger | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | poor effects by Mg2+ and Zn2+ at 5 mM | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger US368 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 14.24fold from culture supernatant by ultrafiltration and gel filtration to homogeneity | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimization of production of beta-glucanase with barley flour as the sole carbon source | Aspergillus niger | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Aspergillus niger | cellotriose + cellotetraose + cellobiose + D-glucose | oligosaccharides (mainly trisaccharide and tetrasaccharide) followed by disaccharide (cellobiose) and glucose are formed as the major products of hydrolysis after a prolonged incubation of 4 h | ? | |
barley beta-glucan + H2O | - |
Aspergillus niger US368 | cellotriose + cellotetraose + cellobiose + D-glucose | oligosaccharides (mainly trisaccharide and tetrasaccharide) followed by disaccharide (cellobiose) and glucose are formed as the major products of hydrolysis after a prolonged incubation of 4 h | ? | |
lichenan + H2O | - |
Aspergillus niger | ? | - |
? | |
lichenan + H2O | - |
Aspergillus niger US368 | ? | - |
? | |
additional information | the enzyme is a conformation-retaining and is only active toward glucan containing beta-1,3-1,4-linkages. No activity with laminarin and carboxymethyl cellulose | Aspergillus niger | ? | - |
? | |
additional information | the enzyme is a conformation-retaining and is only active toward glucan containing beta-1,3-1,4-linkages. No activity with laminarin and carboxymethyl cellulose | Aspergillus niger US368 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x *32000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3;1,4-glucanase | - |
Aspergillus niger |
Lichenase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | activity range, profile overview | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, pH 5.0, half-life is 150 min | Aspergillus niger |
60 | - |
purified enzyme, pH 5.0, half-life is 135 min | Aspergillus niger |
65 | - |
purified enzyme, pH 5.0, half-life is 90 min | Aspergillus niger |
70 | - |
purified enzyme, pH 5.0, half-life is 30 min | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.32 | - |
lichenan | pH 5.0, 60°C | Aspergillus niger | |
19.11 | - |
Barley beta-glucan | pH 5.0, 60°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6.5 | activity range, profile overview | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | purified enzyme, 37°C, 1 h, completely stable | Aspergillus niger |