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Literature summary for 3.2.1.73 extracted from

  • Na, H.B.; Jung, W.K.; Jeong, Y.S.; Kim, H.J.; Kim, S.K.; Kim, J.; Yun, H.D.; Lee, J.K.; Kim, H.
    Characterization of a GH family 8 beta-1,3-1,4-glucanase with distinctive broad substrate specificity from Paenibacillus sp. X4 (2015), Biotechnol. Lett., 37, 643-655.
    View publication on PubMed
Search for more literature for 3.2.1.73

Cloned(Commentary)

Cloned (Comment) Organism
-
 Paenibacillus sp.

Protein Variants

Protein Variants Comment Organism
D156A almost complete loss of activity Paenibacillus sp.
Q95A almost complete loss of activity Paenibacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ increases the activity and thermostability of BGlc8H with substrate lichenan Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
-
 Paenibacillus sp.
41561
-
-
 Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. A0A088BCU2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a signal peptide of 31 amino acids Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.22
-
 substrate barley beta-D-glucan, pH 5.5, 50°C Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-D-glucan + H2O
-
 Paenibacillus sp. ?
-
 ?
carboxymethyl cellulose + H2O 32.2% of the activity with barley beta-D-glucan Paenibacillus sp. ?
-
 ?
cellohexaose + H2O
-
 Paenibacillus sp. cellobiose + cellotriose no release of D-glucose ?
chitosan + H2O 81.3% of the activity with barley beta-D-glucan Paenibacillus sp. ?
-
 ?
laminarin + H2O 4.1% of the activity with barley beta-D-glucan Paenibacillus sp. ?
-
 ?
lichenan + H2O 14.5% of the activity with barley beta-D-glucan Paenibacillus sp. ?
-
 ?
additional information enzyme hydrolyzes mixed-linked beta-1,3-1,4-glucans and chitosan as well as carboxymethyl cellulose, showing activities of EC 3.2.1.6 and EC 3.2.1.74 Paenibacillus sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 41561, calculated, x * 40000, SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
Blc8H
-
 Paenibacillus sp.
Lic8H
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paenibacillus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Paenibacillus sp. calculated
-
 7.6