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Literature summary for 3.2.1.73 extracted from

  • Cheng, R.; Xu, L.; Wang, S.; Wang, Y.; Zhang, J.
    Recombinant expression and characterization of an acid-, alkali- and salt-tolerant beta-1,3-1,4-glucanase from Paenibacillus sp. S09 (2014), Biotechnol. Lett., 36, 797-803.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene plicA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Paenibacillus sp.

General Stability

General Stability Organism
the purified recombinant His-tagged enzyme retains over 90% activity in 4 M NaCl at 25°C for 24 h Paenibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Paenibacillus sp.
Fe2+
-
Paenibacillus sp.
Fe3+
-
Paenibacillus sp.
Mn2+
-
Paenibacillus sp.
SDS about 55% inhibition Paenibacillus sp.
Zn2+
-
Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 3.7 mg/ml at pH 6.2, 55°C with barley beta-glucan, recombinant enzyme Paenibacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activtion, stabilizes the enzyme at higher temperatures Paenibacillus sp.
Co2+ slight activtion Paenibacillus sp.
Mg2+ slight activtion Paenibacillus sp.
additional information EDTA has little effect on enzymatic activity Paenibacillus sp.
NaCl 1.5fold activation at 0.5 M, the enzyme is acid-, alkali- and salt-tolerant, the purified recombinant His-tagged enzyme retains over 90% activity in 4 M NaCl at 25°C for 24 h Paenibacillus sp.
Triton X-100 about 20% activation Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 239000, about, sequence calculation, x * 25000, recombinant His-tagged enzyme, SDS-PAGE Paenibacillus sp.
239000
-
x * 239000, about, sequence calculation, x * 25000, recombinant His-tagged enzyme, SDS-PAGE Paenibacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Paenibacillus sp. the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan ?
-
?
additional information Paenibacillus sp. S09 the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan ?
-
?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. M9XGK2 gene plicA
-
Paenibacillus sp. S09 M9XGK2 gene plicA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 2.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7055
-
purified recombinant His-tagged enzyme, substrate barley beta-glucan, pH 6.2, 55°C Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
Paenibacillus sp. ?
-
?
barley beta-glucan + H2O
-
Paenibacillus sp. S09 ?
-
?
additional information the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan Paenibacillus sp. ?
-
?
additional information enzyme substrate specificity, overview. No activity with laminarin, carboxymethyl cellulose, xylan and soluble starch Paenibacillus sp. ?
-
?
additional information the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan Paenibacillus sp. S09 ?
-
?
additional information enzyme substrate specificity, overview. No activity with laminarin, carboxymethyl cellulose, xylan and soluble starch Paenibacillus sp. S09 ?
-
?
oat beta-glucan + H2O
-
Paenibacillus sp. ?
-
?
oat beta-glucan + H2O
-
Paenibacillus sp. S09 ?
-
?

Subunits

Subunits Comment Organism
? x * 239000, about, sequence calculation, x * 25000, recombinant His-tagged enzyme, SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
beta-1,3-1,4-glucanase
-
Paenibacillus sp.
Lichenase
-
Paenibacillus sp.
PlicA
-
Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Paenibacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 recombinant His-tagged enzyme, activity range, profile, overview Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified recombinant His-tagged enzyme retaining more than 70% activity at up to 50°C for 2 h Paenibacillus sp.
55
-
purified recombinant His-tagged enzyme, half-life is about 50 min, 10% activity remaining after 2 h Paenibacillus sp.
60
-
purified recombinant His-tagged enzyme, inactivation within 10 min, in presence of 5 mM CaCl2 the enzyme retains over 80% activity after 2 h Paenibacillus sp.
65
-
purified recombinant His-tagged enzyme, in presence of 5 mM CaCl2 the enzyme retains 40% activity after 10 min, and 20% after 20 min, inactivation after 1 h Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Paenibacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
3.5 10 recombinant His-tagged enzyme, activity range, profile, overview Paenibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 12 the enzyme is acid-, alkali- and salt-tolerant, the purified recombinant His-tagged enzyme retains more than 80% activity after incubation for 4 h Paenibacillus sp.

General Information

General Information Comment Organism
evolution the enzyme is a member of the glycosylhydrolase family GH16 containing the conserved motif EIDIEF and signal peptide Paenibacillus sp.