Cloned (Comment) | Organism |
---|---|
gene plicA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Paenibacillus sp. |
General Stability | Organism |
---|---|
the purified recombinant His-tagged enzyme retains over 90% activity in 4 M NaCl at 25°C for 24 h | Paenibacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Paenibacillus sp. | |
Fe2+ | - |
Paenibacillus sp. | |
Fe3+ | - |
Paenibacillus sp. | |
Mn2+ | - |
Paenibacillus sp. | |
SDS | about 55% inhibition | Paenibacillus sp. | |
Zn2+ | - |
Paenibacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 3.7 mg/ml at pH 6.2, 55°C with barley beta-glucan, recombinant enzyme | Paenibacillus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activtion, stabilizes the enzyme at higher temperatures | Paenibacillus sp. | |
Co2+ | slight activtion | Paenibacillus sp. | |
Mg2+ | slight activtion | Paenibacillus sp. | |
additional information | EDTA has little effect on enzymatic activity | Paenibacillus sp. | |
NaCl | 1.5fold activation at 0.5 M, the enzyme is acid-, alkali- and salt-tolerant, the purified recombinant His-tagged enzyme retains over 90% activity in 4 M NaCl at 25°C for 24 h | Paenibacillus sp. | |
Triton X-100 | about 20% activation | Paenibacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 239000, about, sequence calculation, x * 25000, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus sp. |
239000 | - |
x * 239000, about, sequence calculation, x * 25000, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Paenibacillus sp. | the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan | ? | - |
? | |
additional information | Paenibacillus sp. S09 | the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | M9XGK2 | gene plicA | - |
Paenibacillus sp. S09 | M9XGK2 | gene plicA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 2.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Paenibacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7055 | - |
purified recombinant His-tagged enzyme, substrate barley beta-glucan, pH 6.2, 55°C | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Paenibacillus sp. | ? | - |
? | |
barley beta-glucan + H2O | - |
Paenibacillus sp. S09 | ? | - |
? | |
additional information | the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan | Paenibacillus sp. | ? | - |
? | |
additional information | enzyme substrate specificity, overview. No activity with laminarin, carboxymethyl cellulose, xylan and soluble starch | Paenibacillus sp. | ? | - |
? | |
additional information | the enzyme specifically catalyzes the hydrolysis of beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in 1,3-1,4-beta-glucan | Paenibacillus sp. S09 | ? | - |
? | |
additional information | enzyme substrate specificity, overview. No activity with laminarin, carboxymethyl cellulose, xylan and soluble starch | Paenibacillus sp. S09 | ? | - |
? | |
oat beta-glucan + H2O | - |
Paenibacillus sp. | ? | - |
? | |
oat beta-glucan + H2O | - |
Paenibacillus sp. S09 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 239000, about, sequence calculation, x * 25000, recombinant His-tagged enzyme, SDS-PAGE | Paenibacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Paenibacillus sp. |
Lichenase | - |
Paenibacillus sp. |
PlicA | - |
Paenibacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Paenibacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | recombinant His-tagged enzyme, activity range, profile, overview | Paenibacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant His-tagged enzyme retaining more than 70% activity at up to 50°C for 2 h | Paenibacillus sp. |
55 | - |
purified recombinant His-tagged enzyme, half-life is about 50 min, 10% activity remaining after 2 h | Paenibacillus sp. |
60 | - |
purified recombinant His-tagged enzyme, inactivation within 10 min, in presence of 5 mM CaCl2 the enzyme retains over 80% activity after 2 h | Paenibacillus sp. |
65 | - |
purified recombinant His-tagged enzyme, in presence of 5 mM CaCl2 the enzyme retains 40% activity after 10 min, and 20% after 20 min, inactivation after 1 h | Paenibacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Paenibacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 10 | recombinant His-tagged enzyme, activity range, profile, overview | Paenibacillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 12 | the enzyme is acid-, alkali- and salt-tolerant, the purified recombinant His-tagged enzyme retains more than 80% activity after incubation for 4 h | Paenibacillus sp. |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the glycosylhydrolase family GH16 containing the conserved motif EIDIEF and signal peptide | Paenibacillus sp. |