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Literature summary for 3.2.1.73 extracted from

  • Hung, Y.L.; Chen, H.J.; Liu, J.C.; Chen, Y.C.
    Catalytic efficiency diversification of duplicate beta-1,3-1,4-glucanases from Neocallimastix patriciarum J11 (2012), Appl. Environ. Microbiol., 78, 4294-4300.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene bglA13, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli Neocallimastix patriciarum
gene bglA16, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli Neocallimastix patriciarum
gene bglA51, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli Neocallimastix patriciarum
gene bglM2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli Neocallimastix patriciarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 0.86 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C Neocallimastix patriciarum
additional information
-
additional information 0.90 mg/ml barley beta-glucan, recombinant enzyme, pH 6.0, 40°C Neocallimastix patriciarum
additional information
-
additional information 0.94 mg/ml with barley beta-glucan, at recombinant enzyme, pH 6.0, 40°C Neocallimastix patriciarum
additional information
-
additional information 1.19 mg/ml with barley beta-glucan, recombinant enzyme, pH 6.0, 40°C Neocallimastix patriciarum

Organism

Organism UniProt Comment Textmining
Neocallimastix patriciarum I3W8N0 BglM2; isolated from the feces of water buffalo, gene bglM2
-
Neocallimastix patriciarum I3W8N1 BglA51; isolated from the feces of water buffalo, gene bglA51
-
Neocallimastix patriciarum I3W8N2 BglA13; isolated from the feces of water buffalo, gene bglA13
-
Neocallimastix patriciarum I3W8N3 BglA16; isolated from the feces of water buffalo, gene bglA16
-
Neocallimastix patriciarum J11 I3W8N0 BglM2; isolated from the feces of water buffalo, gene bglM2
-
Neocallimastix patriciarum J11 I3W8N1 BglA51; isolated from the feces of water buffalo, gene bglA51
-
Neocallimastix patriciarum J11 I3W8N2 BglA13; isolated from the feces of water buffalo, gene bglA13
-
Neocallimastix patriciarum J11 I3W8N3 BglA16; isolated from the feces of water buffalo, gene bglA16
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Neocallimastix patriciarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26530
-
purified recombinant enzyme, pH 6.0, 40°C, substrate barley beta-glucan Neocallimastix patriciarum
32100
-
purified recombinant enzyme, pH 6.0, 40°C, substrate barley beta-glucan Neocallimastix patriciarum
39520
-
purified recombinant enzyme, pH 6.0, 40°C, substrate barley beta-glucan Neocallimastix patriciarum
41210
-
purified recombinant enzyme, pH 6.0, 40°C, substrate barley beta-glucan Neocallimastix patriciarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O best substrate Neocallimastix patriciarum ?
-
?
barley beta-glucan + H2O best substrate Neocallimastix patriciarum J11 ?
-
?
lichenan + H2O
-
Neocallimastix patriciarum ?
-
?
lichenan + H2O
-
Neocallimastix patriciarum J11 ?
-
?
additional information no activity with pachyman, laminarin, starch, carboxymethyl cellulose, Avicel, and xylan Neocallimastix patriciarum ?
-
?
additional information no activity with pachyman, laminarin, starch, carboxymethyl cellulose, Avicel, and xylan Neocallimastix patriciarum J11 ?
-
?

Synonyms

Synonyms Comment Organism
beta-1,3-1,4-glucanase
-
Neocallimastix patriciarum
BglA13
-
Neocallimastix patriciarum
BglA16
-
Neocallimastix patriciarum
BglA51
-
Neocallimastix patriciarum
BglM2
-
Neocallimastix patriciarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Neocallimastix patriciarum
50
-
-
Neocallimastix patriciarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
63.06
-
Barley beta-glucan recombinant enzyme, pH 6.0, 40°C Neocallimastix patriciarum
68.19
-
Barley beta-glucan recombinant enzyme, pH 6.0, 40°C Neocallimastix patriciarum
83.89
-
Barley beta-glucan recombinant enzyme, pH 6.0, 40°C Neocallimastix patriciarum
133.3
-
Barley beta-glucan recombinant enzyme, pH 6.0, 40°C Neocallimastix patriciarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Neocallimastix patriciarum
6.5
-
-
Neocallimastix patriciarum

General Information

General Information Comment Organism
evolution the presence of expansion and several predicted secondary structures in the 3' untranslated regions of genes bglA16 and bglM2 suggest that these two genes were duplicated recently, whereas genes bglA13 and bglA16, which contain very short 3'UTRs, were replicated earlier. The activities and some characteristics of enzymes have changed during the horizontal gene transfer event. Duplicated beta-glucanase genes bglA16 and bglM2 increase the reaction efficiency of beta-glucanases and suggest that the catalytic efficiency of beta-glucanase is likely to be a criterion determining the evolutionary fate of duplicate forms in Neocallimastix patriciarum J11 Neocallimastix patriciarum