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Literature summary for 3.2.1.73 extracted from

  • Wang, J.; Niu, C.; Liu, X.; Chen, X.; Li, Q.
    Characterization of a new 1,3-1,4-beta-glucanase gene from Bacillus tequilensis CGX5-1 (2014), Appl. Biochem. Biotechnol., 173, 826-837.
    View publication on PubMed
Search for more literature for 3.2.1.73

Activating Compound

Activating Compound Comment Organism Structure
SDS activates to 139.72% Bacillus tequilensis
Triton X-100 activates to 221.30% Bacillus tequilensis
Tween-20 activates to 159.20% Bacillus tequilensis
Tween-80 activates to 267.73% Bacillus tequilensis

Application

Application Comment Organism
food industry the thermostable enzyme can be useful in mashing at 72°C of brewing processes Bacillus tequilensis

Cloned(Commentary)

Cloned (Comment) Organism
gene bgl5-1, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 Bacillus tequilensis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Bacillus tequilensis
Ca2+ 16% inhibition at 10 mM, 15% at 1 mM Bacillus tequilensis
Co2+ 6% inhibition at 10 mM, 16% at 1 mM Bacillus tequilensis
DTT
-
 Bacillus tequilensis
EDTA
-
 Bacillus tequilensis
Fe2+ 92% inhibition at 10 mM Bacillus tequilensis
Li+ 48.5% inhibition at 10 mM, 18% at 1 mM Bacillus tequilensis
Mg2+ activates by 89% at 1 mM, inhibits 50% at 10 mM Bacillus tequilensis
Mn2+ 45% inhibition at 10 mM, 20% at 1 mM Bacillus tequilensis
Zn2+ 52% inhibition at 10 mM, 44% at 1 mM Bacillus tequilensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 10.0 mg/ml with barley beta-glucan, pH 6.0, 40°C, recombinant enzyme Bacillus tequilensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates by 89% at 1 mM, inhibits 50% at 10 mM Bacillus tequilensis
additional information metal ions are required for enzyme activity and also disulfide linkage may be involved in catalytic activity Bacillus tequilensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 26900, about, sequence calculation, x * 24000, recombinant enzyme, SDS-PAGE Bacillus tequilensis
26900
-
 x * 26900, about, sequence calculation, x * 24000, recombinant enzyme, SDS-PAGE Bacillus tequilensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
barley beta-glucan + H2O Bacillus tequilensis
-
 ?
-
 ?
barley beta-glucan + H2O Bacillus tequilensis CGX5-1
-
 ?
-
 ?
lichenan + H2O Bacillus tequilensis
-
 ?
-
 ?
lichenan + H2O Bacillus tequilensis CGX5-1
-
 ?
-
 ?
oat beta-glucan + H2O Bacillus tequilensis
-
 ?
-
 ?
oat beta-glucan + H2O Bacillus tequilensis CGX5-1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus tequilensis K0A689 isolated from Asian giant hornet, Vespa mandarinia, nest, gene bgl5-1
-
Bacillus tequilensis CGX5-1 K0A689 isolated from Asian giant hornet, Vespa mandarinia, nest, gene bgl5-1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by dialysis, ultrafiltration, and nickel affinity chromatography Bacillus tequilensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7500
-
 purified recombinant enzyme, substrate barley beta-glucan, pH 6.0, 40°C Bacillus tequilensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Bacillus tequilensis ?
-
 ?
barley beta-glucan + H2O best substrate Bacillus tequilensis ?
-
 ?
barley beta-glucan + H2O
-
 Bacillus tequilensis CGX5-1 ?
-
 ?
barley beta-glucan + H2O best substrate Bacillus tequilensis CGX5-1 ?
-
 ?
lichenan + H2O
-
 Bacillus tequilensis ?
-
 ?
lichenan + H2O
-
 Bacillus tequilensis CGX5-1 ?
-
 ?
additional information no activity with carboxymethyl cellulose, xylan, laminarin, and soluble starch, substrate specificity, overview Bacillus tequilensis ?
-
 ?
additional information no activity with carboxymethyl cellulose, xylan, laminarin, and soluble starch, substrate specificity, overview Bacillus tequilensis CGX5-1 ?
-
 ?
oat beta-glucan + H2O
-
 Bacillus tequilensis ?
-
 ?
oat beta-glucan + H2O
-
 Bacillus tequilensis CGX5-1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 26900, about, sequence calculation, x * 24000, recombinant enzyme, SDS-PAGE Bacillus tequilensis

Synonyms

Synonyms Comment Organism
1,3-1,4-beta-glucanase
-
 Bacillus tequilensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Bacillus tequilensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 high activity within this range, recombinant enzyme Bacillus tequilensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 40 purified recombinant enzyme, pH 6.0, 70 min, completely stable Bacillus tequilensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus tequilensis

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 activity range, over 80% activity within the range of pH 5.0-7.5, recombinant enzyme Bacillus tequilensis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus tequilensis sequence calculation
-
 6.76