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Literature summary for 3.2.1.73 extracted from
- Cloning of LicB from Clostridium thermocellum and its efficient secretive expression of thermostable beta-1,3-1,4-glucanase (2014), Appl. Biochem. Biotechnol., 173, 562-570.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the secretively produced beta-1,3-1,4-glucanase shows excellent thermostability up to 80°C and a wide pH range from pH 4 to pH 11 and has a potential in the food and animal feed applications | Acetivibrio thermocellus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression and secretion from Bacillus subtilis strain WB800 using vector pP43JM2 | Acetivibrio thermocellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibits 37% at 10 mM | Acetivibrio thermocellus |  |
| Fe2+ | inhibits 51% at 10 mM | Acetivibrio thermocellus | |
| Mn2+ | inhibits 6.5% at 10 mM | Acetivibrio thermocellus | |
| additional information | poor inhibition at 10 mM by Co2+ and EDTA | Acetivibrio thermocellus | |
| Zn2+ | inhibits 15% at 10 mM | Acetivibrio thermocellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 1.65 mg/ml with beta-1,3-1,4-glucan, at pH 6.0, 70°C | Acetivibrio thermocellus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effects by 10 mM of Na+, K+, Ca2+, and Mg2+ | Acetivibrio thermocellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,3-1,4-glucan + H2O | Acetivibrio thermocellus | - |
? | - |
? | |
| beta-1,3-1,4-glucan + H2O | Acetivibrio thermocellus DSM 1237 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
| Acetivibrio thermocellus DSM 1237 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,3-1,4-glucan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| beta-1,3-1,4-glucan + H2O | - |
Acetivibrio thermocellus DSM 1237 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-1,4-glucanase | - |
Acetivibrio thermocellus |
| LicB | - |
Acetivibrio thermocellus |
| Lichenase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
recombinant enzyme | Acetivibrio thermocellus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | activity range, recombinant enzyme | Acetivibrio thermocellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
purified recombinant enzyme, pH 7.0, 40% activity remaining after 6 h | Acetivibrio thermocellus |
| 80 | - |
purified recombinant enzyme, pH 7.0, no activity remaining after 1 h | Acetivibrio thermocellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Acetivibrio thermocellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | activity range, recombinant enzyme, profile overview | Acetivibrio thermocellus |
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